Synthesis and Characterization of a Hydroxyethyl Derivative of Chitosan and Evaluation of Its Biosafety

查看全文 作　　者：SHAO [1]Kai;HAN [1]Baoqin;GAO [1]Jinning;SONG [1]Fulai;YANG [1]Yan;LIU [1]Wanshun

机构地区：[1]College of Marine Life Science, Ocean University of China

出　　处：《Journal of Ocean University of China》索引2015年第14卷第4期,共7页

基　　金：supported by the National Key Technology R&D Program of the Ministry of Science and Technology(2013BAB01B02) 摘　　要：Hydroxyethyl chitosan(HE-chitosan) is a water-soluble derivative of chitosan with many apparent biological properties. For example, it is non-toxic and rapidly biodegradable. Moreover, HE-chitosan has advantages in water-solubility, moisture retention and gelling property due to its hydroxyethyl group. However, the biocompatibility and biodegradability of this multifunctional derivative have rarely been documented although they are critical for its application in biomedical and clinical treatments. The purpose of this work was to evaluate the biosafety of HE-chitosan, and draw important clues for its diverse applications. HE-chitosan was synthesized and characterized its chemical structure with FTIR. Its molecular weight(MW) was determined by gel permeation chromatography(GPC), and its deacetylation degree(DD) was investigated through potentiometric analysis. The cytotoxicity of HE-chitosan on mouse fibroblast cell L929 was tested. The biocompatibility and biodegradability of HE-chitosan in rat and rabbit were evaluated. The FTIR results indicated that the hydroxyethyl groups were linked to C6 of chitosan. The GPC analysis confirmed that its Mw was about 90.01 k Da. It was also demonstrated that HE-chitosan had excellent biocompatibility and biodegradability in vivo and had no cytotoxicity on L929. These findings indicated that HE-chitosan can potentially be applied as a biomaterial in tissue engineering, drug delivery, and other biomedical fields. 关键词：水溶性衍生物安全性评价壳聚糖合成表征羟乙基生物降解性生物相容性凝胶渗透色谱法

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Synthesis and Characterization of a Hydroxyethyl Derivative of Chitosan and Evaluation of Its Biosafety

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