Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D

查看全文 作　　者：LU Jin-[1]Jian;LU De-[2]Zhao;CHEN Yu-[2]Fei;DONG Ya-[2]Ting;ZHANG Jun-[2]Ren;LI [1]Ting;TANG Zheng-[1]Hai;YANG [2]Zhen

机构地区：[1]State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macao;[2]College of Life Sciences, Zhejiang Chinese Medical University

出　　处：《Chinese Journal of Natural Medicines》索引2015年第13卷第9期,共7页

基　　金：supported by the Science and Technology Development Fund,Macao S.A.R(FDCT)(070/2013/A);the Research Fund of University of Macao(SRG026-ICMS13-LJJ and MRG008-LJJ2014-ICMS);the Administration of Traditional Chinese Medicine of Zhejiang Province(No.2012 ZA028);Program of Xinmiao Talents in Zhejiang Province(No.2010 R410024) 摘　　要：Platycodin D(PD), a triterpenoid saponin isolated from Platycodonis Radix, is a famous Chinese herbal medicine that has been shown to have anti-proliferative effects in several cancer cell lines. The aim of this study was to determine the changes in cellular proteins after the treatment of hepatocellular carcinoma HepG2 cells with PD using proteomics approaches. The cell viability was determined using the MTT assay. The proteome was analyzed by two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot analysis was used to confirm the expression of changed proteins. Our results showed that PD inhibited the proliferation of HepG2 cells in concentration- and time-dependent manners. Sixteen proteins were identified to be up-regulated in PD-treated HepG2 cells, including ATP5 H, OXCT1, KRT9, CCDC40, ERP29, RCN1, ZNF175, HNRNPH1, HSP27, PA2G4, PHB, BANF1, TPM3, ECH1, LGALS1, and MYL6. Three proteins(i.e., RPS12, EMG1, and KRT1) decreased in HepG2 cells after treatment with PD. The changes in HSP27 and PHB were further confirmed by Western blotting. In conclusion, our results shed new lights on the mechanisms of action for the anti-cancer activity of PD. 关键词：药学药剂学调剂学剂型

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Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D

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