Neuroprotective effect of ethyl acetate extract from gastrodia elata against transient focal cerebral ischemia in rats induced by middle cerebral artery occlusion

查看全文 作　　者：Duan [1]Xiaohua;Wang [2]Weili;Liu [3]Xiaoqing;Yan [2]Hanwen;Dai [2]Rong;Lin [2]Qing

机构地区：[1]the Key Modern Research Laboratory for Ethno-pharmacognosy of Yunnan Higher School,Yunnan University of Traditional Chinese Medicine;[2]the Department of Pharmacology,Yunnan University of Traditional Chinese Medicine;[3]Center for Neuron and Disease,Frontier Institute of Science and Technology,Xi'an Jiaotong University

出　　处：《Journal of Traditional Chinese Medicine》索引2015年第35卷第6期,共8页

基　　金：Supported by Natural Science Foundation of China (Anti-ischemic Brain Injury and Neuroprotection Mechanisms of Phenolic Compounds Distributed in Brain from Gastrodia,No.81160514);Yunnan Applied Basic Research Projects (Protective Effect and Mechanisms of 4-Methoxybenzyl from Gastrodia Elata on the Neurovascular Unit,No.2014FB153);Traditional Chinese Medicine Education Innovation Fund of Yunnan Baiyao-Yunnan University of TCM (The Regulation Role of Ethyl Acetate Extract from Gastrodia Elata on NO Pathway,No.YB2014J04) 摘　　要：OBJECTIVE: To investigate the protective effect of neuroprotection against transient focal cerebral ischemia of the extract from Tianma(Rhizoma Gastrodiae) and the possible mechanisms underlying the action.METHODS: Cerebral ischemia-reperfusion injury was induced through middle cerebral artery occlusion(MCAO). Adult male Sprague-Dawley rats were randomly divided into four groups: sham-operated,ischemia-reperfusion model, 102.6 mg/kg extract treated and 11.4 mg/kg extract treated groups. The extract was prepared from gastrodia elata with ethyl acetate. The effect of the extract tested on rat neurological de fi cits and Cerebral index, cerebral infarct volume, brain injury, terminal dexynucleotidyl transferase-mediated d UTP nick end labeling(TUNEL) and B-cell lymphoma-2(Bcl-2) positive cells.RESULTS: The extract was able to reduce neurological scores, cerebral index and cerebral infarction rate. The brain injury was also relieved by the extract. The results of immunofluorescence staining analysis indicated that the extract increased the expression of Bcl-2 and reduced TUNEL-positive cells significantly in the extract treated groups.CONCLUSION: These results suggested that the extract relieved ischemic injury induced by transient focal cerebral ischemia in rats, and this neuroprotective effect might be partially due to the attenuated apoptosis pathway. 关键词：ETHYL ACETATE Gastrodia Neuroprotec-tive AGENTS REPERFUSION INJURY APOPTOSIS

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Neuroprotective effect of ethyl acetate extract from gastrodia elata against transient focal cerebral ischemia in rats induced by middle cerebral artery occlusion

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