Prussian blue nanoparticle-loaded microbubbles for photothermally enhanced gene delivery through ultrasound-targeted microbubble destruction

查看全文 作　　者：Xiaoda [1]Li;Xiuli [1]Yue;Jinrui [2]Wang;Xiaolong [2]Liang;Lijia [1]Jing;Li [1]Lin;Yongbo [1]Yang;Shanshan [1]Feng;Yajun [2]Qian;Zhifei [2]Dai

机构地区：[1]School of Municipal and Environmental Engineering, School of Life Science and Technology, Harbin Institute of Technology;[2]College of Engineering, Peking University Third Hospital,Peking University

出　　处：《Science Bulletin》索引2016年第61卷第2期,共9页

基　　金：supported by the National Natural Science Foundation of China(81371580 and 21273014);the National Natural Science Foundation for Distinguished Young Scholars(81225011);the State Key Program of National Natural Science of China(81230036) 摘　　要：By adsorbing chitosan(CS)-functionalized Prussian blue(PB) nanoparticles(CS/PB NPs) complexing DNA onto the surface of gas encapsulated microbubbles(MBs), a multifunctional gene delivery system of MBs@CS/PB/DNA was fabricated for photothermally enhanced gene transfection through ultrasound-targeted microbubble destruction. CS/PB NPs of(2.69 ± 0.49) nm could complex DNA effectively when the mass ratio was2:1. It was found that MBs@CS/PB/DNA could enhance ultrasound imaging greatly both in vitro and in vivo. In addition, MBs@CS/PB/DNA could be disrupted by applying a higher-intensity ultrasound irradiation to release CS/PB/DNA, which could effectively transform the nearinfrared(NIR) light into heat to assist the uptake of CS/PB/DNA by cells. With the aid of ultrasound irradiation and NIR light irradiation, the gene transfection efficiency was significantly enhanced to(43.08 ± 1.13) %, much higher than polyethylenimine. Moreover, MBs@CS/PB/DNA showed excellent biocompatibility, encouraging the further exploration of MBs@CS/PB/DNA to be a platform for combined ultrasound image, photothermal therapy, drug delivery, and gene therapy. 关键词：基因传递纳米粒子超声成像普鲁士蓝微泡光热 DNA制备载药

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Prussian blue nanoparticle-loaded microbubbles for photothermally enhanced gene delivery through ultrasound-targeted microbubble destruction

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