Development of Three Multiplex PCR Primer Sets for Ark Shell(Scapharca broughtonii)and Their Validation in Parentage Assignment

查看全文 作　　者：[1]LINing;LI [1]Qi;KONG [1]Lingfeng;YU [1]Hong

机构地区：[1]Key Laboratory of Mariculture of Ministry of Education,Ocean University of China,Qingdao 266003,P.R.China

出　　处：《Journal of Ocean University of China》索引2016年第15卷第2期,共7页

基　　金：supported by National Marine Public Welfare Research Program(201205023);National Natural Science Foundation of China(41276138);Doctoral Program of Ministry of Education of China(2013 0132110009) 摘　　要：Scapharca broughtonii is a commercially important and over-exploited species.In order to investigate its genetic diversity and population structure,43 novel polymorphic microsatellites were isolated and characterized.The number of alleles per locus ranged from 3 to 22 with an average of 6.93,and the observed and expected heterozygosities varied between 0.233 and 1.000,and 0.250 and 0.953,with an average of 0.614 and 0.707,respectively.Three highly informative multiplex PCRs were developed from nine of those microsatellites for S.broughtonii.We evaluated and validated these multiplex PCRs in 8 full-sib families.The average polymorphism information content(PIC) was 0.539.The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns.Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets.Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S.broughtonii.The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction,population genetic analysis and brood stock management. 关键词：多重PCR PCR引物验证微卫星位点等位基因数群体遗传学分配亲子

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Development of Three Multiplex PCR Primer Sets for Ark Shell(Scapharca broughtonii)and Their Validation in Parentage Assignment

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