Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae

查看全文 作　　者：Qing-Ji [1]Shen;Hakimi [1]Kassim;Yong [1,2]Huang;Hui [1,3]Li;Jing [1]Zhang;Guang [4]Li;Peng-Ye [3]Wang;Jun [4]Yan;Fangfu [3]Ye;Ji-Long [1]Liu

机构地区：[1]Medical Research Council Functional Genomics Unit,Department of Physiology,Anatomy and Genetics,University of Oxford;[2]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University;[3]Key Laboratory of Soft Matter Physics,Beijing National Laboratory for Condensed Matter Physics,Institute of Physics,Chinese Academy of Sciences;[4]CAS-MPG Partner Institute for Computational Biology,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences

出　　处：《Journal of Genetics and Genomics》索引2016年第43卷第6期,共12页

基　　金：supported by the UK Medical Research Council (to J.L.L.), China Scholarship Council-University of Oxford Scholarship (to Q.J.S), Chinese Scholarship Council Studentship (to Y.H.), Malaysia Government Scholarship (to H.K.), the National Natural Science Foundation of China (No. 11304372) (to H.L., F. Y and P.Y.W.) and anonymous donation (to J.L.L.) 摘　　要：Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filamentforming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frameGFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases(Ura7p and Ura8p) and two asparagine synthetases(Asn1p and Asn2p) form filaments both in the cytoplasm and in the nucleus.Live imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in S. cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated. 关键词：CTP synthase Metabolic enzyme Cytoophidium GLYCOLYSIS GLUTAMINE Intracellular compartmentation Saccharomyces cerevisiae

相关文献

参考文献（59）
引证文献（3）
耦合文献（32）

参考文献(59)

引证文献(3)

耦合文献(32)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae

参考文献(59)

引证文献(3)

耦合文献(32)

用户提示