Hippo/YAP signaling pathway is involved in osteosarcoma chemoresistance

查看全文 作　　者：Dong-Yu [1]Wang;Ya-Nan [1]Wu;Jun-Qi [1]Huang;Wei [1]Wang;Meng [1]Xu;Jin-Peng [1]Jia;Gang [1]Han;Bei-Bei [2]Mao;Wen-Zhi [1]Bi

机构地区：[1]Department of Orthopaedics and Rehabilitation,PLA General Hospital;[2]State Key Laboratory of Brain and Cognitive Sciences,Institute of Biophysics,Chinese Academy of Sciences

出　　处：《Chinese Journal of Cancer》索引2016年第35卷第7期,共8页

基　　金：supported by the National Natural Science Foundation of China(No.81172553 and 81472513 to WB) 摘　　要：Background:Osteosarcoma is the most common bone malignancy in children and adolescents,and 20%-30%of the patients suffer from poor prognosis because of individual chemoresistance.The Hippo/yes-associated protein(YAP) signaling pathway has been shown to play a role in tumor chemoresistance,but no previous report has focused on its involvement in osteosarcoma chemoresistance.This study aimed to investigate the role of the Hippo/YAP signaling pathway in osteosarcoma chemoresistance and to determine potential treatment targets.Methods:Using the Cell Titer-Glo Luminescent cell viability assay and flow cytometry analysis,we determined the proliferation and chemosensitivity of YAP-overexpressing and YAP-knockdown osteosarcoma cells.In addition,using western blotting and the real-time polymerase chain reaction technique,we investigated the alteration of the Hippo/YAP signaling pathway in osteosarcoma cells treated with chemotherapeutic agents.Results:Mammalian sterile 20-like kinase 1(MST1) degradation was increased,and large tumor suppressor kinase1/2(LAT51/2) total protein levels were decreased by methotrexate and doxorubicin,which increased activation and nuclear translocation of YAP.Moreover,YAP increased the proliferation and chemoresistance of MG63 cells.Conclusions:The Hippo/YAP signaling pathway plays a role in osteosarcoma chemoresistance,and YAP is a potential target for reducing chemoresistance. 关键词：肿瘤耐药信号通路骨肉瘤河马化疗流式细胞仪分析聚合酶链反应技术信号转导通路

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Hippo/YAP signaling pathway is involved in osteosarcoma chemoresistance

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