Comparison of concentration methods for detection of hepatitis A virus in water samples

查看全文 作　　者：Yuting [1,2]Qiao;Zhiwei [2]Sui;Guoliang [3]Hu;Huabin [3]Cao;Guoxiang [4]Yang;Yong [4]Li;Yongsong [4]Lei;Lihua [1]Zhao;Quanjiao [1]Chen

机构地区：[1]CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;[2]National Institute of Metrology, Beijing 100013, China;[3]College of Animal Science and Technology, Institute of Animal Population Health, Jiangxi Agricultural University, Nanchang 330045, China;[4]Hubei Wildlife Epidemic Focus and Disease Surveillance Center, Wuhan 430075, China

出　　处：《Virologica Sinica》索引2016年第31卷第4期,共8页

基　　金：supported by grants from the National Science and Technology Support Program (No.2013BAK12B05);the National Basic Research Program (No.2010CB530301);the National Natural Science Foundation of China (No.31070141,31100132) 摘　　要：Hepatitis A virus is a pathogen associated with water pollution.Contaminated drinking water can cause hepatitis A outbreaks,lead to economic losses,and even threaten human lives.It is difficult to detect low levels of hepatitis A virus in water,so the virus must be concentrated in order to quantify it accurately.Here,we present a simple,rapid,efficient technique for the concentration and detection of hepatitis A virus in water.Our data showed that adding phosphate-buffered saline to the water,pre-filtering the water,and adding Trizol reagent directly to the filtration membrane can significantly improve concentration efficiency.Of three types of filtration membranes studied(mixed cellulose ester membrane,polyvinylidene fluoride membrane,and nylon membrane),the concentration efficiency using mixed cellulose ester membrane with a 0.1-μm pore size was the highest,reaching 92.62 ± 5.17%.This method was used to concentrate hepatitis A virus in water samples from Donghu Lake.Using SYBR Green real-time reverse transcription polymerase chain reaction analysis,the detection sensitivity of this method reached 10~1 copies/μL and its concentration efficiency reached 79.45 ± 9.88%. 关键词：CONCENTRATION filtration membrane hepatitis A virus mixed cellulose ester(MCE) membrane water samples

相关文献

参考文献（29）
引证文献（1）
耦合文献（7）

参考文献(29)

引证文献(1)

耦合文献(7)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Comparison of concentration methods for detection of hepatitis A virus in water samples

参考文献(29)

引证文献(1)

耦合文献(7)

用户提示