Effects of Inoculum Density on Plant Growth and Hydrocarbon Degradation

查看全文 作　　者：Ghulam [1]SHABIR;Muhammad [2]ARSLAN;Kaneez [1]FATIMA;Imran [1]AMIN;Qaiser Mahmood [1]KHAN;Muhammad [1]AFZAL 机构地区：[1]Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE);[2]Earth Sciences Department, King Fahd University of Petroleum and Minerals 出　　处：《Pedosphere》索引2016年第26卷第5期,共5页 基　　金：supported by the Higher Education Commission (HEC), Pakistan (No. 20-2011-1997) 摘　　要：The combined use of plants and bacteria is a promising approach for the remediation of soil contaminated with organic pollutants.Different biotic and abiotic factors can affect the survival and activity of the applied bacteria and consequently plant growth and phytoremediation efficiency. The effect of inoculum density on the abundance and expression of alkane-degrading genes in the rhizosphere of plant vegetated in hydrocarbon-contaminated soil has been rarely observed. In this study, an alkane-degrading bacterium(Pantoea sp. strain BTRH79), at different inoculum densities(10~5 to 10~8 cells cm^(-3) soil), was inoculated to ryegrass(Lolium perenne) vegetated in diesel-contaminated soil to find the optimum inoculum density needed for its efficient colonization and hydrocarbon degradation activity. Bacterial inoculation improved plant growth and hydrocarbon degradation. Maximum plant growth and hydrocarbon degradation were observed with the inoculum having the highest cell density(10~8 cells cm^(-3) soil). Moreover, the inoculum with higher cell density exhibited more abundance and expression of alkane hydroxylase gene, CYP153. This study suggests that the inoculum density is one of the main factors that can affect bacterial colonization and activity during phytoremediation. 关 键 词：接种密度 植物生长 烃降解菌 非生物因素 污染土壤 细胞密度 油气 物理修复