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Characterization of a collection of Chinese fir elite genotypes using sequence.related amplified polymorphism markers

查看全文 作　　者：Hongjing [1,2]Duan;Dehuo [2]Hu;Yun [1]Li;Huiquan [2]Zheng 高影响力作者 机构地区：[1]National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China;[2]Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and Pest, Guangdong Academy of Forestry,Guangzhou 510520, People's Republic of China高影响力机构 出　　处：《Journal of Forestry Research》索引2016年第27卷第5期,共6页高影响力期刊 基　　金：supported by the National Natural Sciences Foundation of China(No.31200506);the Special Fund for Forest Scientific Research in the Public Welfare(No.201404127);the Special Plan for the Cultivation of High-level Talents of Guangdong(No.2014TQ01N140);the National‘863’Project of the 12th Five-Year Plan in China(2011AA100203) 摘　　要：Chinese fir [Cunninghamia lanceolata(Lamb.)Hook] is a major timber tree species in China.In the present study,sequence-related amplified polymorphism(SRAP) markers were used to evaluate the polymorphisms,diversity,and relationships in a collection of Chinese fir elite genotypes(n=103) from the Lechang provenance,an endangered provenance.Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 non-Lechang provenance genotypes;577(93.1 %) of these were polymorphic,yielding an average of 16.5 polymorphic bands per primer combination.Of the SRAP primer combinations(n=35),14 ones identified C70.0 % of the 120 genotypes.The Me12–Em19 combination had the strongest discriminatory ability with 93.3 % of the genotypes identified.An UPGMA dendrogram further showed that most of the Lechang provenance genotypes(87.4 %) grouped together(Cluster A).The Lechang genotypes had a considerable amount of genetic variation with genetic similarity from 0.40 to 0.81.The current work may facilitate the management of the endangered Lechang Chinese fir provenance. 关 键 词：优良基因型 多态性标记 杉木种源 收集 UPGMA聚类分析 鉴定 SRAP 扩增多态性
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