Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants

查看全文 作　　者：Kimiaki [1]Tanabe;Yuko [1]Hojo;Tomonori [1]Shinya;Ivan [1]galis

机构地区：[1]Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan

出　　处：《Journal of Integrative Plant Biology》索引2016年第58卷第11期,共11页

基　　金：supported by Grant-in-Aid for Scientific Research (No. 24570026, IG; No. 15K18820, TS);Japan Advanced Plant Science Network that provided the LC-MS/MS instrumentation for metabolite measurements 摘　　要：Two phenolamides(PAs), p-coumaroylputrescine and feruloylputrescine strongly accumulate in rice(Oryza sativa cv. Nipponbare) leaves subjected to attack of chewing and sucking herbivores. Here we identified and characterized in vitro three novel rice genes that mediated coumaroyl-Co A/feruloyl-Co A conjugation to polyamines, putrescine and agmatine. Interestingly, two genes were highly specific for their polyamine substrates, encoding putrescine N-hydroxycinnamoyltransferase and agmatine N-hydroxycinnamoyltransferase, while the third enzyme could use both polyamines and it was therefore annotated as putrescine/agmatine N-hydroxycinnamoyltransferase. All genes were preferentially expressed in rice roots and developing flowers,and in addition, the putrescine/agmatine N-hydroxycinnamoyltransferase transcripts were strongly induced by wounding in the young rice leaves. Because the wound response of this gene was only partially suppressed in the jasmonoyl-L-isoleucine deficient plants(Osjar1), it suggests that its upregulation(as well as inducible PAs in rice) may be largely independent of jasmonoyl-L-isoleucine signaling pathway. The finding of three closely related genes with a similar and/or overlapping activity in PA biosynthesis provides another striking example of rapid diversification of plant metabolism in response to environmental stresses in nature. 关键词：水稻植株生物合成生化变化基因介导胍基丁胺证据分子水稻叶片

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Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants

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