Identification of interaction between HIV-1 glycoprotein 41 and integrase

查看全文 作　　者：Xiaowei [1]Zhang;Fei [3]Zhang;Xiaohe [1]Ma;Xing [1]Zhao;Wei [1]Li;Zhiping [1]Zhang;Jibin [3]Zhang;Xian-En [2]Zhang;Zongqiang [1]Cui 机构地区：[1]State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China;[2]National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;[3]College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China 出　　处：《Virologica Sinica》索引2016年第31卷第5期,共10页 基　　金：supported by the National Nano Project(grant no.2011CB933600);the National Natural Science Foundation of China(grant no.31470269);supported by the Institute of Biophysics and the National Laboratory of Biomacromolecules at the Chinese Academy of Sciences(CAS) 摘　　要：Human immunodeficiency virus-1(HIV-1) encodes 15 viral proteins. Protein-protein interactions play a large role in the function of these proteins. In this study, we attempted to identify novel interactions between the HIV-1 proteins to better understand the role played by viral protein-protein interactions in the life cycle of HIV-1. Genes encoding the 15 viral proteins from the HIV-1 strain AD8 were inserted into the plasmids of a yeast two-hybrid system. By screening 120 pairs of proteins, interactions between seven pairs were found. This led to the discovery of an interaction between the HIV-1 proteins integrase(IN) and glycoprotein 41(gp41), which was confirmed by both co-immunoprecipitation(Co-IP) assays and fluorescence resonance energy transfer(FRET)imaging in live cells. In addition, it was found that the amino acids at positions 76–100 of gp41 are required for it to bind to IN. Deletion of this region from gp41 prevented its interaction with IN and reduced the production of HIV-1 in 293 T cells. This study provides new information on HIV-1protein-protein interactions which improves the understanding of the biological functions of gp41 and IN during the virus life cycle. 关 键 词：人的免疫不全 virus-1 (HIV-1 ) glycoprotein 41 (gp41 ) integrase (在里面) 蛋白质蛋白质相互作用 酵母二混血儿的试金