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De novo transcriptome analysis of Spodoptera exigua multiple nucleopolyhedrovirus(SeMNPV) genes in latently infected Se301 cells

查看全文 作　　者：Zheng [1]Fang;Jingxu [1]Shao;Qingbei [1]Weng 高影响力作者 机构地区：[1]School of Life Sciences, Guizhou Normal University高影响力机构 出　　处：《Virologica Sinica》索引2016年第31卷第5期,共12页高影响力期刊 基　　金：funded by the National Natural Science Foundation of China (No. 31160378);the Science and Technology Foundation of Guizhou Province (No. LKS[2010]21);the Startup Foundation for Doctors of Guizhou Normal University 摘　　要：Cells of the P8-Se301-C1 strain are Spodoptera exigua cell clones that each harbor a partial version of the S. exigua multiple nucleopolyhedrovirus(SeMNPV) genome and which are resistant to homologous SeMNPV infections. The cells produce no viral progeny, suggesting that the infection is a latent-like viral infection. To investigate the SeMNPV genes harbored in the P8-Se301-C1 cells,the de novo transcriptomes of P8-Se301-C1 cells and S. exigua Se301 cells were analyzed and compared. A total of 54,569,296 reads were obtained from the P8-Se301-C1 cells that yielded112,565 final unigenes with a mean length of 1,093 nt. A total of 56,865,504 reads were obtained from the Se301 cells that yielded 102,996 final unigenes with a mean length of 1,082 nt. Ten SeMNPV gene transcripts(se5, se7, se8, se12, se43, se45, se89, se90, se124, and se126) were detected in the P8-Se301-C1 cells by RNA-Seq but not in the Se301 cells, which was verified by RTPCR. 5'/3' RACE analyses showed that the 3'- or 5'-end sequences of the viral transcripts are aligned to the host gene sequences in P8-Se301-C1 cells, suggesting that the SeMNPV genes may integrate into and be transcribed with the host genes in the P8-Se301-C1 cells. Furthermore, six additional viral gene transcripts, se11, se42, se44, se88, se91, and se127(incorporated into chimeric fusion transcripts in the P8-Se301-C1 cells), were detected in the RACE analyses. Taken together, sixteen SeMNPV transcripts were identified in the P8-Se301-C1 cell strain. This study provides information to develop the understanding of baculovirus latent infections and superinfection exclusion. 关 键 词：RNA-SEQ SeMNPV BACULOVIRUS 潜伏的感染 Spodoptera exigua
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