Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model

查看全文 作　　者：Qin [1,2]Wei;Yeping [3]Bian;Fuchao [1]Yu;Qiang [2]Zhang;Guanghao [1]Zhang;Yang [1,2]Li;Songsong [1]Song;Xiaomei [4]Ren;Jiayi [1,2]Tong

机构地区：[1]Cardiovascular Institute,Southeast University;[2]Department of Cardiology,Zhongda Hospital Affiliated to Southeast University;[3]Department of Intensive Care Unit,Jiangsu Province Official Hospital;[4]Department of Geriatrics,Zhongda Hospital Affiliated to Southeast University

出　　处：《The Journal of Biomedical Research》索引2016年第30卷第6期,共6页

基　　金：supported by Medical Key Talents Foundation of Jiangsu Province,China(No:904-KJXW18);by National Natural Science Youth Foundation of China(No.81300227 and No.81300159) 摘　　要：Chronic intermittent hypoxia is considered to play an important role in cardiovascular pathogenesis during the development of obstructive sleep apnea(OSA).We used a well-described OSA rat model induced with simultaneous intermittent hypoxia.Male Sprague Dawley rats were individually placed into plexiglass chambers with air pressure and components were electronically controlled.The rats were exposed to intermittent hypoxia 8 hours daily for 5weeks.The changes of cardiac structure and function were examined by ultrasound.The cardiac pathology,apoptosis,and fibrosis were analyzed by H&E staining,TUNNEL assay,and picosirius staining,respectively.The expression of inflammation and fibrosis marker genes was analyzed by quantitative real-time PCR and Western blot.Chronic intermittent hypoxia/low pressure resulted in significant increase of left ventricular internal diameters(LVIDs),endsystolic volume(ESV),end-diastolic volume(EDV),and blood lactate level and marked reduction in ejection fraction and fractional shortening.Chronic intermittent hypoxia increased TUNNEL-positive myocytes,disrupted normal arrangement of cardiac fibers,and increased Sirius stained collagen fibers.The expression levels of hypoxia induced factor(HIF)-l α,NF-κB,IL-6,and matrix metallopeptidase 2(MMP-2) were significantly increased in the heart of rats exposed to chronic intermittent hypoxia.In conclusion,the left ventricular function was adversely affected by chronic intermittent hypoxia,which is associated with increased expression of HIF-lα and NF-κB signaling molecules and development of cardiac inflammation,apoptosis and fibrosis. 关键词：OBSTRUCTIVE sleep APNEA MODEL chronic intermittent hypoxia CARDIAC DYSFUNCTION INFLAMMATION

相关文献

参考文献（19）
引证文献（17）
耦合文献（7）

参考文献(19)

引证文献(17)

耦合文献(7)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model

参考文献(19)

引证文献(17)

耦合文献(7)

用户提示