MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica

查看全文 作　　者：LI [1,2]Po;GONG Xiao-[1]dong;JIA [1]Hui;FAN Yong-[1,3]shan;ZHANG Yun-[1,3]feng;CAO Zhi-[1]yan;HAO Zhi-[1]min;HAN Jian-[1]min;GU Shou-[1]qin;DONG Jin-[1]gao

机构地区：[1]Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071000, P.R. China;[2]Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Baoding 071000, P.R. China;[3]Tangshan Normal University, Tangshan 063000, P.R. China

出　　处：《Journal of Integrative Agriculture》索引2016年第15卷第12期,共9页

基　　金：supported by the National Natural Science Foundation of China (31171805 and 31371897) 摘　　要：The mitogen-activated protein kinase(MAPK), a key signal transduction component in the MAPK cascade pathway, regulates a variety of physiological activities in eukaryotes. However, little is known of the role MAPK plays in phytopathogenic fungi. In this research, we cloned the MAPK gene STK1 from the northern corn leaf blight pathogen Setosphaeria turcica and found that the gene shared high homology with the high osmolality glycerol(HOG) MAPK gene HOG1 of Saccharomyces cerevisiae. In addition, gene knockout technology was employed to investigate the function of STK1. Gene knockout mutants(KOs) were found to have altered hyphae morphology and no conidiogenesis, though they did show similar radial growth rate compared to the wild-type strain(WT). Furthermore, microscope observations indicated that STK1 KOs did not form normal appressoria at 48 h post-inoculation on a hydrophobic surface. STK1 KOs had reduced virulence, a significantly altered Helminthosporium turcicum(HT)-toxin composition, and diminished pathogenicity on the leaves of susceptible inbred corn OH43. Mycelium morphology appeared to be significantly swollen and the radial growth rates of STK1 KOs declined in comparison with WT under high osmotic stress. These results suggested that STK1 affects the hyphae development, conidiogenesis, and pathogenicity of S. turcica by regulating appressorium development and HT-toxin biosynthesis. Moreover, the gene appears to be involved in the hypertonic stress response in S. turcica. 关键词：MAPK级联途径玉米大斑病菌植物病原真菌菌丝形态高渗透压毒素成分分生孢子生物合成

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MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica

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