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Quercetin protects liver injury induced by bile duct ligation via attenuation of Rac1 and NADPH oxidase1 expression in rats

查看全文 作　　者：Razieh [1]Kabirifar;Zohreh-al-sadat [1]Ghoreshi;Fatemeh [2]Safari;Alireza [3]Karimollah;Ali [1]Moradi;Ebrahim Eskandari-[4]nasab 高影响力作者 机构地区：[1]Department of Biochemistry,School of Medicine,Shahid Sadoughi University of Medical Sciences and Health Services,Yazd,Iran;[2]Department of Physiology,School of Medicine,Shahid Sadoughi University of Medical Sciences and Health Services,Yazd,Iran;[3]Department of Pharmacology,School of Medicine,Shahid Sadoughi University of Medical Sciences and Health Services,Yazd,Iran;[4]Department of Biochemistry,School of Medicine,Zahedan University of Medical Sciences,Zahedan,Iran高影响力机构 出　　处：《Hepatobiliary & Pancreatic Diseases International》索引2017年第16卷第1期,共8页高影响力期刊 摘　　要：BACKGROUND: Bile duct ligation(BDL) and subsequent cholestasis are correlated with oxidative stress, hepatocellular injury and fibrosis. Quercetin is a flavonoid with antifibrotic,and hepatoprotective properties. However, the molecular mechanism underlying quercetin-mediated hepatoprotection is not fully understood. The current study was to evaluate mechanisms of hepatoprotective effect of quercetin in BDL rat model.METHODS: We divided male Wistar rats into 4 groups(n=8 for each): sham, sham+quercetin(30 mg/kg per day), BDL, and BDL+quercetin(30 mg/kg per day). Four weeks later, the rats were sacrificed, the blood was collected for liver enzyme measurements and liver for the measurement of Rac1, Rac1-GTP and NOX1 mR NA and protein levels by quantitative PCR and Western blotting, respectively.RESULTS: Quercetin significantly alleviated liver injury in BDL rats as evidenced by histology and reduced liver enzymes.Furthermore, the m RNA and protein expression of Rac1,Rac1-GTP and NOX1 were significantly increased in BDL rats compared with those in the sham group(P<0.05); quercetin treatment reversed these variables back toward normal(P<0.05). Another interesting finding was that the antioxidant markers e.g. superoxide dismutase and catalase were elevated in quercetin-treated BDL rats compared to BDL rats(P<0.05).CONCLUSION: Quercetin demonstrated hepatoprotective activity against BDL-induced liver injury through increasing antioxidant capacity of the liver tissue, while preventing the production of Rac1, Rac1-GTP and NOX1 proteins. 关 键 词：WISTAR大鼠 急性肝损伤 槲皮素 保护作用 NADPH 结扎 诱导 胆管
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