Nat Methods:利用CRISPR-Cas9组合筛选发现癌症弱点

查看全文 作　　者：John Paul Shen, Ana Bojorquez-Gomez, Katherine Licon, Kristin Klepper, Daniel Pekin, Alex N Beckett, Kyle Salinas Sanchez, Jason F Kreisberg, Trey [1]Ideker;John Paul Shen, Trey Ideker, Prashant [2]Mali;John Paul Shen, Dongxin Zhao, Dan Du, Assen Roguev, Jason F Kreisberg, Nevan Krogan, Lei Qi, Trey Ideker, Prashant [3]Mali;Dongxin Zhao, Chih-Chung Kuo, Prashant [4]Mali;Roman Sasik, Amanda Birmingham, Aaron N Chang, Trey [5]Ideker;Jens Luebeck, Alex [6]Thomas;Alex Thomas, Chih-Chung Kuo, Nathan E [7]Lewis;Dan [8]Du;Assen Roguev, Nevan [9]Krogan;Nathan E [10]Lewis;Lei [11]Qi 机构地区：[1]Department of Medicine, Division of Genetics, University of California, San Diego, La Jolla, California, USA;[2]Moores UCSD Cancer Center, La Jolla, California, USA;[3]The Cancer Cell Map Initiative (CCMI), La Jolla and San Francisco, California, USA;[4]Department of Bioengineering, University of California, San Diego, La Jolla, California, USA;[5]Center for Computational Biology & Bioinformatics, University of California, San Diego, La Jolla, California, USA;[6]Bioinformatics & Systems Biology Program, University of California, San Diego, La Jolla, California, USA;[7]Novo Nordisk Center for Biosustainability at the University of California, San Diego, La Jolla, California, USA;[8]Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA;[9]Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA;[10]Department of Pediatrics, University of California, San Diego, La Jolla, California, USA;[11]Department of Bioengineering, Stanford University, Stanford, California, USA. 出　　处：《现代生物医学进展》索引2017年第17卷第17期,共1页 摘　　要：导致癌症的基因突变也会削弱癌细胞,从而使得人们有机会开发选择性地杀死它们同时不影响正常细胞的药物。这一概念被称作”合成致死性(synthetic lethality)”,这是因为这些药物仅对发生突变的(或者说合成的)细胞是致命性的。在一项新的研究中。来自美国加州大学圣地亚哥分校、加州大学旧金山分校、斯坦福大学和癌细胞图谱项目(Cancer Cell Map Initiative)的研究人员开发出一种新的方法来寻找合成致死性的基因组合。 关 键 词：组合筛选 癌症 美国加州大学 利用 基因突变 斯坦福大学 癌细胞 正常细胞