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Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato

查看全文 作  者:JIN [1]Na;XUE [1]Hui;LI Wen-[1]jing;WANG Xue-[1]yan;LIU [1]Qian;LIU Shu-[1]sen;LIU [1]Pei;ZHAO Jian-[1]lon;JIAN [1]Heng 高影响力作者 机构地区:[1]Department of Plant Pathology, China Agricultural University/Key Laboratory of Plant Pathology, Ministry of Agriculture, Beijing 100193, P.R.China高影响力机构 出  处:《Journal of Integrative Agriculture》索引2017年第16卷第6期,共11页高影响力期刊 基  金:financially supported by the National Basic Research Program of China (2013CB127501);the National Key Research and Development Program of China (2016YFD0201003);the National Natural Science Foundation of China (31272021, 31571987) 摘  要:Streptomyces rubrogriseus HDZ-9-47,isolated from eggs of Meloidogyne spp.,was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field.Microscopic observations showed that HDZ-9-47 parasitized eggs of M.incognita within 7 days.In vitro,the culture filtrate of HDZ-9-47 caused 97.0%mortality of second-stage juveniles(J2s)of M.incognita and inhibited more than 50%egg hatching.In the field,compared with the control,the rootknot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7%at 90 days post transplantation,which were better than that in other application doses and methods.In addition,reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%,respectively,better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment.And tomato yield also increased by 16.1%.Together,our results suggest that HDZ-9-47 could be an effective biocontrol agent of M.incognita,and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M.incognita control. 关 键 词:Meloidogyne n Streptomyces rubrogriseus BIOCONTROL 寄生状态 nematicidal 代谢物 控制功效
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