Refinement of Methodology for Cadmium Determination in Soil Micro-Arthropod Tissues

查看全文 作　　者：ZHU [1,2]Dong;KE [3]Xin;WU [1]Longhua;HUANG [1]Yujuan;Peter [1]CHRISTIE;LUO [4]Yongming

机构地区：[1]Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences;[2]University of Chinese Academy of Sciences;[3]Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences;[4]Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences

出　　处：《Pedosphere》索引2017年第27卷第3期,共11页

基　　金：supported by the National High Technology Research and Development Program of China (No. 2012AA101402-2);the National Natural Science Foundation of China (Nos. 41271264, 41325003) 摘　　要：Highly precise and reliable determination of heavy metals in soil micro-arthropod tissues remains a challenge because of the small size of the animals and their typical low abundance in metal-contaminated agricultural soils. The present study sought to develop a method for cadmium(Cd) determination in soil micro-arthropods by optimizing the sample digestion procedure, reducing sample weight, modifying sample pre-treatment and validating the methodology with field samples. The optimized digestion conditions comprised a sample mass of 50–150 μg, digestion reagent of nitric acid:hydrogen peroxide(3:1), digestion temperature of 105℃,digestion period of 3 h and digestion volume of 30 μL. Defecation of the standard Collembola Folsomia candida(92 h) and the indigenous Collembola Onychiurus yodai(42 h) and ultrasonic cleaning of F. candida increased the accuracy of Cd determination.The recovery of Cd using the refined procedure was 98.9% and the limits of detection and quantification were 0.002 and 0.008 μg L^(-1), respectively. The within-batch precision values were < 3%. The Cd concentrations in the tissues of the Collembola Isotoma sp.collected from a range of metal-contaminated fields determined by the improved method were consistent with the Cd concentrations in the field soils. The results indicate that the optimized method can be used for more accurate or reliable determination of Cd concentrations in soil micro-arthropod tissues. 关键词：镉(CD) 污染土壤测定方法动物组织消化温度样品消解重金属含量样品前处理

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Refinement of Methodology for Cadmium Determination in Soil Micro-Arthropod Tissues

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