Construction of Oryza sativa genome contigs by fingerprint strategy

查看全文 作　　者：TAO QUANZHOU;YUEMIN QIAN;HAIYING ZHAO;SHULIANG YU;LONGFANG QIU;BOQIAN WU;JIA ZHU;DI YU;XIAOHUI LIU;GUOFAN HONG(National Center for Gene Research, Chinese Academy of Sciences, Shanghai 200233, China.)(Shanghai Institute of Biochemistry, Chinese Acad 出　　处：《Cell Research》索引1995年第5卷第2期,共9页 摘　　要：We described the construction of BAC contigs of the genome of a indica variety of Oryza sativa, Guang Lu Ai 4.An entire representative (sixfold coverage of rice chromosomes) and genetically stable BAC library of rice genome constructed in this lab has been systematically analysed by restriction enzyme fragmentation and polyacrylamide gel electrophoresis. And all the images thus obtained were subject to image-processing, which consisted of preliminary location of bands, cooperative tracking of lanesby correlation of adjacent bands, a precise densitometric pass, alignment at the marker bands with the standard,optional interactive editing, and normalization of the accepted bands. The contigs were generated based on the Computer Software specially designed for genome map ping. The number of contigs with 600 kb in length on average was 464; of contigs with 1000 kb in length on average was 107; of contigs with 1500 kb in length on average was 23. Therefore, all the contigs we have obtained amounted up to 420 megabases in length. Considering the size of rice genome (430 megabased), the contigs generated in this lab have covered nearly 98% of the rice genome. We are now in the process of mapping the contigs to chromosomes. 关 键 词：水稻 基因组 Contigs图 指纹技术 遗传连锁图 作图