In vivo genome editing thrives with diversified CRISPR technologies

查看全文 作　　者：Xun [1]Ma;Avery Sum-Yu [1]Wong;Hei-Yin [1]Tam;Samuel Yung-Kin [1]Tsui;Dittman Lai-Shun [1]Chung;Bo [1,2,3]Feng

机构地区：[1]Key Laboratory for Regenerative Medicine in Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong;[2]Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences;[3]SBS Core Laboratory, CUHK Shenzhen Research Institute

出　　处：《Zoological Research》索引2018年第39卷第2期,共14页

基　　金：supported by funds provided by the Research Grants Council of Hong Kong(CUHK 14104614,TBF16ENG007 and TBF17MED002 to B.F.;and 3132966 to W.Y.C.);funds from the Croucher Foundation(CAS16CU01/CAS16401 to W.Y.C.);the National Basic Research Program of China(973 Program,2015CB964700 to Y.L.) 摘　　要：Prokaryotic type Ⅱ adaptive immune systems have been developed into the versatile CRISPR technology, which has been widely applied in sitespecific genome editing and has revolutionized biomedical research due to its superior efficiency and flexibility. Recent studies have greatly diversified CRISPR technologies by coupling it with various DNA repair mechanisms and targeting strategies.These new advances have significantly expanded the generation of genetically modified animal models, either by including species in which targeted genetic modification could not be achieved previously, or through introducing complex genetic modifications that take multiple steps and cost years to achieve using traditional methods. Herein, we review the recent developments and applications of CRISPR-based technology in generating various animal models, and discuss the everlasting impact of this new progress on biomedical research. 关键词：多样化染色体技术 VIVO 编辑生物医学动物模型原核生物

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In vivo genome editing thrives with diversified CRISPR technologies

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