OTS_1-dependent deSUMOylation increases tolerance to high copper levels in Arabidopsis

查看全文 作　　者：Erbao [1]Zhan;Huapeng [1]Zhou;Sha [1]Li;Lei [2]Liu;Tinghong [1]Tan;Honghui [1]Lin

机构地区：[1]Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University;[2]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University

出　　处：《Journal of Integrative Plant Biology》索引2018年第60卷第4期,共13页

基　　金：supported by the National Transgenic Major Project of China (2016ZX08009-003-002 to H.L.);the National Natural Science Foundation of China (31600201 to H.Z; 31470342 and 31670235 to H.L.);the National Basic Research Program of China (2015CB150100 to H.L) 摘　　要：The conjugation of SUMO(small ubiquitin-like modifier) to protein substrates is a reversible process(SUMOylation/deSUMOylation) that regulates plant development and stress responses. The essential metal copper(Cu) is required for normal plant growth, but excess amounts are toxic. The SUMO E_3 ligase, SIZ_1, and SIZ_1-mediated SUMOylation function in plant tolerance to excess Cu. It is unknown whether deSUMOylation also contributes to Cu tolerance in plants. Here, we report that OTS_1, a protease that cleaves SUMO from its substrate proteins,participates in Cu tolerance in Arabidopsis thaliana(Arabidopsis). OTS_1 loss-of-function mutants(ots_(1-2) and ots_(1-3))displayed increased sensitivity to excess Cu. Redox homeostasis and the balance between SUMOylation and deSUMOylation were disrupted in the ots_(1-3) mutant under excess Cu conditions. The ots_(1-3) mutant accumulated higher levels of Cu in both shoots and roots compared to wild type.Specific Cu-related metal transporter genes were upregulated due to the loss-of-function of OTS_1, which might explain the high Cu levels in ots_(1-3). These results suggest that the SUMOylation/deSUMOylation machinery is activated in response to excess Cu, and modulates Cu homeostasis and tolerance by regulating both Cu uptake and detoxification. Together, our findings provide insight into the biological function and regulatory role of SUMOylation/deSUMOylation in plant tolerance to Cu. 关键词：ARABIDOPSIS 植物生长铜层氧化还原作用可逆过程生物功能调查结果蛋白质

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OTS_1-dependent deSUMOylation increases tolerance to high copper levels in Arabidopsis

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