Subcellular Localization of Small GTP-binding Protein DsRab in Dunaliella salina

查看全文 作　　者：Yuting [1]CONG;Jinrong [1]YUE;Zhenyu [1]XING;Xiangnan [1]GAO;Xiujuan [1]LI;Xiaojie [1]CHAI

机构地区：[1]Dalian Ocean University,Key Laboratory of Hydrobiology in Liaoning Province

出　　处：《Agricultural Biotechnology》索引2018年第7卷第3期,共4页

基　　金：Supported by National Natural Science Foundation of China(31472260) 摘　　要：With the total RNA of Dunaliella salina as a template,the cD NA sequence of D. salina small GTP-binding protein gene was amplified by RT-PCR technique,and cloned onto pM Dl8-T simple vector,the recon was subjected to PCR detection and restriction endonuclease analysis,and the total sequence of DNA was determined. The results showed that the cloned fragment was 612 bp,and shared 100% homology with reported D. salina DsRab gene( GenB ank: JN989548). The target gene fragment was inserted downstream of pM DCG 35 S promoter,constructing subcellular localization recombinant vector pM DCG-DsRab. The successfully constructed subcellular localization recombinant vector pM DCG-DsRab was transformed into Agrobacterium tumefaciens LBA4404,and positive single clones were screened and used for transinfection of onion epidemical cells by Agrobacterium-mediated method,and the instant expression of DsRab was observed under fluorescence microscope. The results showed that the fusion protein GFP-DsRab was successfully expressed in onion epidemical cells,and mainly distributed on cytomembrane. This study will provide reference for further illumination of the function and action mechanism of D. salina small GTP-binding protein DsRab. 关键词：蛋白质 GTP 约束力本地化 RT-PCR 盐目标基因荧光显微镜

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Subcellular Localization of Small GTP-binding Protein DsRab in Dunaliella salina

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