Isolation and Identification of Selenium-tolerant Strains from Selenium-rich Soil in Guangxi

查看全文 作　　者：Qing [1]LIAO;Panxia [1]LIANG;Ying [1]XING;Taiqing [1]HUANG;Yongxian [1]LIU;Zepu [1]JIANG

机构地区：[1]Agricultural Resource and Environment Research Institute,Guangxi Academy of Agricultural Sciences

出　　处：《Agricultural Biotechnology》索引2018年第7卷第3期,共5页

基　　金：Supported by the Program for the Scientific Research and Technology Development in Guangxi(Guikehe 415104001-22);the Science and Technology Planning Project of Guangxi(Guikegong 1598006-5-13);the Experiment Station for Selenium Featured Crops in Guangxi(Gui TS2016011);the Fund for Science and Technology Development and Scientific Research of Guangxi Academy of Agricultural Sciences(Guinongke 2015JM23,2017JM01,2017JM03);the Innovation-Driven Development Special Fund Project of Guangxi(Guike AA17202019-1&Guike AA17202019-4) 摘　　要：To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were used to screen the selenium-tolerant strains from the soils which were sampled from the main Selenium-rich areas such as Yongfu,Bama,Yulin Hanshan,Guiping and Tengxian. The results showed that 8 strains with high selenium tolerance were obtained,which could tolerate the selenium concentration above 10 000 μg/m L in solid medium. Among the 8 strains,YLB1-33 showed the highest selenium tolerance,and it could still grow weakly in the solid medium with selenium concentration of 29 000 μg/m L. The sequencing of 16 S rD NA and phylogenetic analysis showed that YLB1-6 was identified as Bacillus cereus,BMB2-1 and TXB1-8 were identified as Bacillus pumilus,GPB2-5 was identified as Bacillus thuringiensis,YLB1-26 and YLB1-33 were identified as Bacillus licheniformis,and YLB1-2 and YFB1-8 were identified as Serratia marcescens. The finding of selenium-tolerance strains had potential application value on promoting the utilization of selenium soil resources and the development of selenium-rich agricultural products in Guangxi. 关键词：土壤屏蔽硒鉴定资源利用种系发生应用程序农业产品土壤资源

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Isolation and Identification of Selenium-tolerant Strains from Selenium-rich Soil in Guangxi

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