Toxicity of silicon dioxide nanoparticles with varying sizes on the cornea and protein corona as a strategy for therapy

查看全文 作　　者：Dayu [1,2,3]Sun;Linji [4,5]Gong;Jing [2,3]xie;Xianliang [2,3]Gu;Yijian [2,3]Li;Qinglin [2,3]Cao;Qiyou [2,3]Li;Luodan [2,3]A.;Zhanjun [4,5]Gu;Haiwei [2,3]Xu

机构地区：[1]Department of Physiology, Third Military Medical University (Army Medical University). Chongqing 400038, China;[2]Southwest Hospital/Southwest Eye Hospital,Third Military Medical University(Army Medical University),Chongqing 400038, China;[3]Key Laboratory of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038, China;[4]Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;[5]University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Science Bulletin》索引2018年第63卷第14期,共10页

基　　金：supported by the National Key R&D program of China(2018YFA0107302,2016YFA0201600);the National Natural Science Foundation of China(81570890);the Foundation of Southwest Hospital(SWH2016LHYS-03) 摘　　要：The human cornea is exposed directly to particulate matter(PM) in polluted air. This exposure can cause eye discomfort and corneal injury. Ultrafine PM(diameter <100 nm) is thought to be particularly harmful to health, but there is limited research investigating its toxicity to the eye. In this study, we evaluated toxicity differences among 30-, 40-, 100-and 150-nm silicon dioxide nanoparticles(SiO_2 NPs) on the cornea. A 24-hour in vitro exposure of primary human corneal epithelial cells(hCECs) to ultrafine(30 and 40 nm) SiO_2 NPs produced toxicity, as evidenced by cell membrane damage, reduced cell viability,increased cell death and mitochondrial dysfunction. In vivo exposure to the same nanoparticles produced observable corneal injury. These effects were more severe with ultrafine than with fine(100 and 150 nm)SiO_2 NPs. Common antioxidant compounds, e.g., glutathione, did not protect the cornea from SiO_2 NP-induced damage. However, foetal bovine serum(FBS) did significantly reduce toxicity, likely by forming a protective protein corona around the nanoparticles. This finding suggests that FBS(or its derivatives) may be a useful clinical therapy for corneal toxicity caused by ultrafine particulates. 关键词：二氧化物蛋白质角膜毒性日冕治疗尺寸硅

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Toxicity of silicon dioxide nanoparticles with varying sizes on the cornea and protein corona as a strategy for therapy

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