Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection

查看全文 作　　者：Thaina Garbino dos [1]Santos;Mery Stefani Leivas [1]Pereira;Diogo Losch [1]Oliveira

机构地区：[1]Laboratory of Cellular Neurochemistry, Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre RS 90035-003, Brazil

出　　处：《Neuroscience Bulletin》索引2018年第34卷第5期,共6页

基　　金：supported by Coordenacao de Aperfeicoamento de Pessoal de Nível Superior(CAPES);Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)-Edital Doencas Neurodegenerativas;Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul(FAPERGS);Financiadora de Estados e Projetos(FINEP) 摘　　要：Drugs that lack the ability to cross the bloodbrain barrier(BBB) need to be placed directly into the central nervous system. Our laboratory studies the involvement of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebellomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solution. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies. 关键词：注射液体鼠脑啮齿类动物治疗神经系统设备修理动物模型

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Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection

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