Genetic diversity of atoxigenic Aspergillus flavus isolates from peanut kernels in China

查看全文 作　　者：Liying [1]Yan;Yanping [1]Kang;Yong [1]Lei;Liyun [1]Wan;Dongxin [1]Huai;Huifang [1]Jiang;Xiaoping [1]Ren;Boshou [1]Liao

机构地区：[1]Key Laboratory of Biology and Genetics Improvement of Oil Crops,Ministry of Agriculture,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences

出　　处：《Oil Crop Science》索引2018年第3卷第1期,共8页

基　　金：supported by the Special Program for Basic Research of the Ministry of Science and Technology (2013FY113400);Chinese Agriculture Research System under Grant No. CARS-14;the National Natural Science Foundation of China under Grant No. 31371662 and 31464443022 摘　　要：Aflatoxin contamination in peanuts is a major threat to public health, especially in tropical and subtropical regions of the world. Recently, the use of atoxigenic Aspergillus flavus strains as biological control agents prove to be effective in reducing aflatoxin contamination in crops. A total of 208 atoxigenic A. flavus isolates, collected from peanut kernels, were grouped into 7 deletion patterns by quadruplex PCR products of nor-1, ver-1, aflR and omtA genes. 49 SNPs, found in 1254 bp fragment of omtA gene, showed genetic variation of omtA among different A. flavus isolates. These isolates were assigned to either MAT1-1 type or MAT1-2 type with primers for each MAT locus. Thus, rich genetic diversity was found in the atoxigenic A. flavus isolates of peanut in China. The results indicated that quadruplex PCR would be an effective method for rapid screening of atoxigenic isolates with gene deletion in aflatoxin biosynthetic cluster. 关键词：PEANUT ASPERGILLUS FLAVUS atoxigenic ISOLATES GENETIC diversity

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Genetic diversity of atoxigenic Aspergillus flavus isolates from peanut kernels in China

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