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Effects of Seed Viability and Number of Generations on Genetic Integrity of Soybean Germplasm Zhonghuang 18 by AFLP Markers

查看全文 作　　者：Dong [1]WANG;Xiaodong [1]ZHANG;Runfang [1]LI;Lingyun [1]LU;Xiaomu [1]WANG;Xiaohong [1]GU;Xia [2]XIN;Guangkun [2]YIN;Xinxiong [2]LU;Hanfeng [1]DING 高影响力作者 机构地区：[1]Shandong Center of Crop Germplasm Resources/Shandong Observation and Experiment Station of Crop Gene Resource & Germplasm Enhancement, Ministry of Agriculture;[2]Institute of Crop Sciences, Chinese Academy of Agricultural Sciences高影响力机构 出　　处：《Agricultural Biotechnology》索引2019年第8卷第2期,共8页高影响力期刊 基　　金：Supported by Key Project of the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period(2013BAD01B0106);the Special Scientific Research Fund of Shandong Academy of Agricultural Sciences for Young Scholars(2016YQN19);China Agriculture Research System-Green Manure(CARS-22);National Crop Germplasm Resources Platform of China(2012/2013-032);Major Scientific and Technological Innovation Project of Shandong Province(2017CXGC0311);Modern Agricultural Industry Technology System of Shandong Province(SDAIT-15-01);Special Fund of Shandong Academy of Agricultural Sciences for Innovation in Agricultural Science and Technology(CXGC2018E15) 摘　　要：The seeds of a soybean cultivar Zhonghuang 18 were subjected to accelerated aging for 0(population G_0-1), 112(population G_0-2), 154(population G_0-3) and 196 d(population G_0-4), whose germination percentage was found to be 98.0%, 95.0%, 81.0%, and 79.0%, respectively. Then, the four populations were regenerated twice in the field. The first descendant populations were marked as G_1-1, G_1-2, G_1-3 and G_1-4, and the second were marked as G_2-1, G_2-2, G_2-3 and G_2-4, respectively. The genetic variation between the control population(G_0-1) and the experimental populations was analyzed using 12 AFLP primer combinations. The results showed that there was no significant difference in genetic similarity between the 11 experimental populations and the control population G_0-1. The genetic similarity between population G_2-4 and G_0-1 was still as high as 0.933 3, indicating that the F_2 generation of the population whose germination percentage was only 79.0% still had a high genetic similarity to the control population. The results of t-tests revealed that the populations G_1-1, G_2-1, G_1-2 and G_2-2 showed no significant difference from the control population G_0-1 in effective number of alleles per locus(Ae), genetic diversity index(H) and Shannon's diversity index(I), while these indices of populations G_0-3, G_0-4, G_1-3, G_1-4, G_2-3 and G_2-4 were significantly reduced. χ~2 tests indicated that the populations G_1-1 and G_2-1 showed little difference, and the populations G_0-2, G_0-3, G_0-4, G_1-2, G_1-3, G_1-4, G_2-2, G_2-3, and G_2-4 showed great difference in allele frequency distribution from the control population G_0-1, and the difference was greater when the seed viability was lower. Compared with the control population G_0-1, the number of rare alleles(Nr) of the populations G_0-2, G_1-1, G_2-1, G_1-2 and G_2-2 showed no significant difference, while that of the populations G_0-3, G_0-4, G_1-3, G_1-4, G_2-3 and G_2-4 declined obviously. These results revealed that compared with the control population, the genetic diversity and Nr for the descendant populations of the populations with 98.0% and 95.0% germination percentages did not change significantly, but declined greatly for the descendant populations of the populations with 81.0.% and 79.0% percentages. The results suggested that the decline in seed viability has a greater impact than the number of generations on genetic structure of soybean germplasm. So, it is suggested that soybean seed with an initial germination percentage of 98.0% should be regenerated before its germination percentage declines to 81.0%. 关 键 词：Soybean SEED VIABILITY NUMBER of generations AFLP Genetic INTEGRITY
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