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Tissue Culture and Rapid Propagation of Spathiphyllum kochii Engl. et Krause

查看全文 作　　者：Shufang [1]FAN;Furong [1]MAO;Bin [2]LIU;Dawei [3]JIAN;Xiaoqin [1]LI;Xuezhi [1]ZUO 高影响力作者 机构地区：[1]College of Biological Engineering and Institute of Plant Germplasm Resources Exploitation and Utilization, Jingchu University of Technology;[2]Institute of Forestry Product Industry, Jiangxi Academy of Forestry;[3]Jingmen Forestry Bureau高影响力机构 出　　处：《Agricultural Biotechnology》索引2019年第8卷第2期,共4页高影响力期刊 基　　金：Supported by Research Project of Jingmen Science and Technology Bureau(YDKY2017008) 摘　　要：[Objectives] This study was conducted on tissue-culture rapid propagation techniques of Spathiphyllum kochii Engl. et Krause. [Methods] With lateral buds of S. kochii as explants, the effects of such four basic media as MS, B5, Nitsch and Wpm and the ratio of two hormones(1, 2, 3 mg/L 6-BA and 0.1, 0.3, 0.5 mg/L NAA) on bud proliferation of S. kochii were studied by the complete test method, and the effect of the ratio of the two hormones(0.25, 0.50, 1.00 mg/L NAA and 1.0, 1.5 mg/L IBA) on rooting of S. kochii as well as the effect of substrate ratio(perlite and peat soil at 1∶9, 2∶8, 3∶7, 4∶6 and 5∶5) on its transplanting survival rate were also studied. [Results] The best basic medium for the rapid propagation of S. kochii was MS. The best hormone ratio suitable for bud proliferation was 2 mg/L6-BA+0.1 mg/L NAA, and the average number of buds proliferated at 45 d was 3.04. The average bud height was 2.05 cm; the most suitable medium for rooting was 1/2 MS+1 mg/L IBA+0.25 mg/L NAA, and its rooting rate was 100%; and the best transplanting substrate was 5∶5 perlite and peat. The soil ratio had a survival rate of 94%. [Conclusions] This study provides a theoretical basis for the improvement of the transplanting survival rate of test-tube S. kochii plantlets. 关 键 词：Spathiphyllum kochii Engl. ET Krause TISSUE CULTURE RAPID PROPAGATION
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