Synchronous detection of glutathione/hydrogen peroxide for monitoring redox status in vivo with a ratiometric upconverting nanoprobe

查看全文 作　　者：Judun [1,2]Zheng;Yunxia [1,2]Wu;Da [1,2]Xing;Tao [1,2]Zhang 机构地区：[1]MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China;[2]College of Biophotonics, South China Normal University, Guangzhou 510631, China 出　　处：《Nano Research》索引2019年第12卷第4期,共8页 基　　金：National Natural Science Foundation of China (Nos. 21771065 and 81630046);Natural Science Foundation of Guangdong Province, China (No. 2017A020215088);Science and Technology Planning Project of Guangdong Province, China (Nos. 2015B020233016 and 2014B020215003);Science and Technology Planning Project of Guangdong Province (Guangdong- Hong Kong Joint Innovation Project), China (No. 2014B050504009) Pearl River Nova Program of Guangzhou, Guangdong Province, China (No. 201806010189);Scientific and Technological Planning Project of Guangzhou, Guangdong Province, China (No. 201805010002). 摘　　要：Cellular redox status presents broad implications with diverse physiological and pathological processes. Simultaneous detection of both the oxidative and reductive species of redox couples, especially the most representative pair glutathione/hydrogen peroxide (GSH/H2O2), is crucial to accurately map the cellular redox status. However, it still remains challenging to synchronously detect GSH/H2O2 in vivo due to lack of a reliable measuring tool. Herein, a ratiometric nanoprobe (UCNP-TB) possessing simultaneous delectability of GSH/H2O2 is established based on a multi-spectral upconverti ng nano phosphor (UCNP-OA) as the lumin esce nee res onance energy tran sfer (LRET) donor and two dye molecules as the acceptors, including a GSH-sensitive dye (TCG) and a H2O2-sensitive dye (BCH). With the as-prepared UCNP-TB, real-time and synchronous monitoring the variation of GSH and H2O2 in vitro and in living mice can be achieved using the ratio of the upcon versi on lumin esce nee (UCL) at 540 and 650 nm to that at 800 nm as the detecti on sign al, respectively, providi ng highly inhere nt reliability of the sensing results by self-calibrati on. Moreover, the nan oprobe is capable of mappi ng the redox status within the drug-resista nt tumor and the drug-induced hepatotoxic liver via ratiometric UCL imaging. Thus, this nan oprobe would provide a reliable tool to elucidate the redox state in vivo. 关 键 词：redox status GLUTATHIONE hydrogen PEROXIDE UPCONVERSION imaging NANOPROBE