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Denitrification and microbial community in MBBR using A. donax as carbon source and biofilm carriers for reverse osmosis concentrate treatment

查看全文 作  者:Li [1,2,3]Li;Guokai [1,2,3]Yan;Haiyan [1,2,3]Wang;Zhaosheng [1,3,4]Chu;Zewen [1,2,3]Li;Yu [1,2,3]Ling;Tong [1,2,3]Wu 高影响力作者 机构地区:[1]State Key Laboratory of Environmental Criteria And Risk As sessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China;[2]Engineering Center for Environmental Pollution Control,Chinese Research Academy of Environmental Sciences,Beijing 100012,China;[3]National Engineering Laboratory for Lake Pollution Control and Ecological Restoration,Chinese Research Academy of Environmental Sciences,Beijing 100012,China;[4]State Environmental Protection Key Laboratory for Lake Pollution Control,Chinese Research Academy of Environmental Sciences,Beijing 100012,China高影响力机构 出  处:《Journal of Environmental Sciences》索引2019年第31卷第10期,共11页高影响力期刊 基  金:supported by the National Major Science and Technology Program for Water Pollution Control and Treatment (Nos. 2017ZX07401003-05-01; 2014ZX07216-001);the China Scholarship Council Foundation (No. 2011911098) 摘  要:In this study,raw Arundo donax(A.donax)pieces were applied as carbon source and biofilm carriers for denitrification in a lab-scale moving bed biofilm reactor(MBBR)for the treatment of reverse osmosis concentrate gathered from local wastewater reuse plant.At stable phase(about 60 days),efficient denitrification performance was obtained with73.2%±19.5%NO3--N average removal and 8.10±3.45 g N/(m3·day)NO3--N average volumetric removal rate.Mass balance analysis showed that 4.84 g A.donax was required to remove 1 g TN.Quantitative real-time PCR analysis results showed that the copy numbers of 16S r-RNA,narG,nirS,nosZ and anammox gene of carrier biofilm and suspended activated sludge in the declination phase(BF2 and AS2)were lower than those of samples in the stable phase(BF1 and AS1),and relatively higher copy numbers of nirS and nirK genes with lower abundance of narG and nosZ genes were observed.High-throughput sequencing analysis was conducted for BF2 and AS2,and similar dominant phyla and classes with different abundance were obtained.The class Gammaproteobacteria affiliated with the phylum Proteobacteria was the most dominant microbial community in both BF2(52.6%)and AS2(41.7%).The PICRUSt prediction results indicated that 33 predictive specific genes were related to denitrification process,and the relative abundance of 18 predictive specific genes in BF2 were higher than those in AS2. 关 键 词:Arundo donax carbon source DENITRIFICATION MBBR Reverse osmosis CONCENTRATE NITRATE removal Microbial community HIGH-THROUGHPUT sequencing
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