Effect of mucin 4 allele on susceptibility to experimental infection with enterotoxigenic F4 Escherichia coli in pigs fed experimental diets

查看全文 作　　者：Samantha [1]O.Sterndale;Danica [1]J.Evans;Josephine [1]P.Mansfield;Julie [2]Clarke;Shafi [3]Sahibzada;Sam [3]Abraham;Mark O’[3]Dea;David [1]W.Miller;Jae Cheol [1,4]Kim;John [1]R.Pluske

机构地区：[1]Agriculture Sciences,College of Science,Health,Engineering and Education Murdoch University,Murdoch,WA 6150,Australia;[2]CSIRO Health and Biosecurity,PO BOX 10041,Adelaide,BC 5000,Australia;[3]Antimicrobial Resistance and Infectious Disease Laboratory,Murdoch University,Murdoch,WA 6150,Australia;[4]AB Vista Asia Pte.Ltd,The Mezzo,Whampoa 329682,Singapore

出　　处：《Journal of Animal Science and Biotechnology》索引2019年第10卷第4期,共9页

基　　金：Australian Pork Limited and the Cooperative Research Centre for High Integrity Australian Pork(Pork CRC)for funding this study;an Australian Pork Limited Postgraduate Scholarship 摘　　要：Background: This study investigated the validity of the DNA-marker based test to determine susceptibility to ETECF4 diarrhoea by comparing the results of two DNA sequencing techniques in weaner pigs following experimental infection with F4 enterotoxigenic Escherichia coli(ETEC-F4). The effects of diet and genetic susceptibility were assessed by measuring the incidence of piglet post-weaning diarrhoea(PWD), faecal E. coli shedding and the diarrhoea index.Results: A DNA marker-based test targeting the mucin 4 gene(MUC4) that encodes F4 fimbria receptor identified pigs as either fully susceptible(SS), partially or mildly susceptible(SR), and resistant(RR) to developing ETEC-F4 diarrhoea. To further analyse this, DNA sequencing was undertaken, and a significantly higher proportion of C nucleotides was observed for RR and SR at the Xba I cleavage site genotypes when compared to SS. However, no significant difference was found between SR and RR genotypes. Therefore, results obtained from Sanger sequencing retrospectively allocated pigs into a resistant genotype(MUC4–), in the case of a C nucleotide, and a susceptible genotype(MUC4+), in the case of a G nucleotide, at the single nucleotide polymorphism site. A total of 72 weaner pigs(age ~ 21 days), weighing 6.1 ± 1.2 kg(mean ± SEM), were fed 3 different diets:(i) positive control(PC) group supplemented with 3 g/kg zinc oxide(Zn O),(ii) negative control(NC) group(no Zn O or HAMSA),and(iii) a diet containing a 50 g/kg high-amylose maize starch product(HAMSA) esterified with acetate. At days five and six after weaning, all pigs were orally infected with ETEC(serotype O149:F4;toxins LT1, ST1, ST2 and EAST). The percentage of pigs that developed diarrhoea following infection was higher(P = 0.05) in MUC4+ pigs compared to MUC4– pigs(50% vs. 26.8%, respectively). Furthermore, pigs fed Zn O had less ETEC-F4 diarrhoea(P = 0.009) than pigs fed other diets, however faecal shedding of ETEC was similar(P > 0.05) between diets.Conclusion: These results confirm that MUC4+ pigs have a higher prevalence of ETEC-F4 diarrhoea following exposure, and that pigs fed Zn O, irrespective of MUC4 status, have reduced ETEC-F4 diarrhoea. Additionally,sequencing or quantifying the single nucleotide polymorphism distribution at the Xba I cleavage site may be more reliable in identifying genotypic susceptibility when compared to traditional methods. 关键词：DIARRHOEA ESCHERICHIA coli F4 MUC4 Weaner PIGS Zinc oxide

相关文献

参考文献（35）
引证文献（0）
耦合文献（40）

参考文献(35)

耦合文献(40)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Effect of mucin 4 allele on susceptibility to experimental infection with enterotoxigenic F4 Escherichia coli in pigs fed experimental diets

参考文献(35)

耦合文献(40)

用户提示