Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

查看全文 作　　者：Joao [1,2,3]BCosta;Joana Silva-[1,2]Correia;Sandra [1,2]Pina;Alain da Silva [1,2]Morais;Sílvia [1,2]Vieira;Hélder [1,2,4,5]Pereira;Joao Espregueira-[1,2,6,7,8]Mendes;Rui [1,2,3]LReis;Joaquim [1,2,3]M.Oliveira

机构地区：[1]B′s Research Group,I3Bs-Research Institute on Biomaterials,Biodegradables and Biomimetics,University of Minho,Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine,AvePark,Zona Industrial da Gandra,4805-017 Barco Gmr,Portugal;[2]ICVS/3B’s-PT Government Associate Laboratory,Braga/Guimaraes,Portugal;[3]The Discoveries Centre for Regenerative and Precision Medicine,Headquarters at University of Minho,Avepark,4805-017 Barco,Guimar's,Portugal;[4]Ripoll y De Prado Sports Clinic:Murcia-Madrid FIFA Medical Centre of Excellence,Murcia,Spain;[5]Orthopedic Department Centro Hospitalar Póvoa de Varzim,Vila do Conde,Portugal;[6]Clínica do Dragao,Espregueira-Mendes Sports Centre,FIFA Medical Centre of Excellence,Porto,Portugal;[7]Dom Henrique Research Centre,Porto,Portugal;[8]Orthopedic Department,University of Minho,Braga,Portugal

出　　处：《Bio-Design and Manufacturing》索引2019年第2卷第4期,共17页

摘　　要：The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patient-speci fic approach. 关键词：PATIENT-SPECIFIC Indirect printing HIERARCHICAL SILK fibroin Enzymatic-cross-linking MENISCUS

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Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

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