Functional Characterization of the Group Ⅰ Alphabaculovirus Specific Gene ac73

查看全文 作　　者：Wei [1,2]Shao;Lihong [1,2]He;Qingxiu [1]Chen;Jiang [1]Li;Fei [1]Deng;Hualin [1]Wang;Zhihong [1]Hu;Manli [1]Wang

机构地区：[1]State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China;[2]University of the Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Virologica Sinica》索引2019年第34卷第6期,共11页

基　　金：supported by the grants from the Key Research Project of Frontier Science (QYZDJ-SSWSMC021);the Strategic Priority Research Program (grant No. XDB11030400) from the Chinese Academy of Sciences;the grants (No. 31621061) from the National Natural Science Foundation of China 摘　　要：Baculoviridae is a family of large DNA viruses that specifically infect insects.It contains four genera,Alpha-,Beta-,Gamma-,and Deltabaculovirus.Alphabaculovirus is further divided into Group I and II,and Group I appears to be emerged most recently among all baculoviruses.Interestingly,there are 12 Group I specific genes that are only found in this lineage.Studying these genes is helpful to understand how baculoviruses evolved.Here,we reported the functional analyzing results of ac73y a function unknown Group I specific gene of Autographa califomica multiple nucleopolyhedrovirus(AcMNPV)which is the type species of baculovirus.The AC73 protein encoded by ac73 was found to be expressed during the late stage of infection and incorporated into the nucleocapsids of budded virus(BV)and occlusionderived virus(ODV).In infected cells,AC73 resided mainly in the ring zone region of the nucleus,and appeared to be assembled into occlusion bodies(OBs).The ac73 knockout and repaired viruses were constructed and studied by in vitro and in vivo infection.Although ac73 was not essential for BV and ODV or OB formation,the BV titer and viral infectivity in insect larvae of ac73 knockout AcMNPV decreased by about 5-8 and 3-4 fold compared to those of wild type virus,respectively,suggesting ac73 contributed to infectious BV production and viral infectivity in vivo.This research provides new insight into the function of this Group I specific gene. 关键词：BACULOVIRUS AC73 Group I NUCLEOCAPSID BcI-2-associated athanogene(BAG)domain

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Functional Characterization of the Group Ⅰ Alphabaculovirus Specific Gene ac73

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