Preparation of a Salmonella LAMP Detection Kit

查看全文 作　　者：Yuping [1,2]WAN;Xiaosheng [1,2]WU;Yu [1,2]ZHANG;Meihong [3]DU;Zhaoqin [1,2]WANG;Caiwei [1,2]FENG;Zhengmiao [1,2]ZHAO;Fangyang [1,2]HE

机构地区：[1]Beijing Kwinbon Biotechnology Co.,Ltd.,Beijing 102206,China;[2]Beijing Engineering Technology Research Center of Food Safety Immune Rapid Detection,Beijing 102206,China;[3]Beijing center for physical&chemical analysis,Beijing 100089,China

出　　处：《Agricultural Biotechnology》索引2019年第8卷第6期,共4页

基　　金：Supported by Guangxi Key R&D Program(GK AB16380271) 摘　　要：[Objectives] This study was conducted to establish a method for rapid detection of Salmonella at the grassroots level, and a corresponding product was developed. [Methods] By analyzing the invA gene of Salmonella, two pairs of efficient, rapid and specific amplification primers were designed and screened for the six conserved domains of the gene, and the LAMP detection kit for Salmonella based on this method was produced. [Results] The method is simple, sensitive, rapid and specific. The minimum detection concentration is 10~2-10~3 CFU/ml. [Conclusions] It can be used in the self-inspection of food production enterprises and the on-site sampling inspection and rapid detection of large quantities of samples by grassroots food safety inspection and supervision departments. 关键词：LAMP SALMONELLA Rapid detection

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Preparation of a Salmonella LAMP Detection Kit

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