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Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium

查看全文 作  者:Saihua [1,3]HUANG;Deyin [3]HUANG;Qitang [1]WU;Meifang [2,3]HOU;Xiaoyan [2,3]TANG;Jian [2]ZHOU 高影响力作者 机构地区:[1]College of Natural Resources and Environment,South China Agricultural University,483 Wushan Street,Guangzhou 510642,China;[2]School of Ecological Technology and Engineering,Shanghai Institute of Technology,100 Haiquan,Shanghai 201418,China;[3]Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control,Guangdong Institute of Eco-environmental and Soil Sciences,808 Tianyuan Road,Guangzhou 510650,China高影响力机构 出  处:《Pedosphere》索引2020年第30卷第2期,共8页高影响力期刊 基  金:supported by the National Natural Science Foundation of China(Nos.41171250,20907011,and 41401356);the Natural Science Foundation of Guangdong Province,China(No.2014A030313702)。 摘  要:Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants(POPs).In this study,effect of environmental C/N ratio on the activities of lignin-degrading enzymes,lignin peroxide(Li P)and manganese peroxidase(Mn P),produced by Phanerochaete chrysosporium,a white rot fungus,was investigated.Glucose was used as C source,and ammonium tartrate of different concentrations was used as N source to provide different C/N ratios.Relationships between Li P and Mn P activities and environmental C/N ratio were explored.The results showed that the higher the N source concentration,the faster the mycelium pellets aged.The faster the mycelium dry weight increased,the higher the Li P and Mn P activities.A high C/N ratio was a necessary condition for the secretion of Li P or Mn P.In addition,mycelium dry weight essentially affected enzyme activities.In the 122 C/N ratio and 50 C/N ratio treatments,mycelium dry weight essentially affected Mn P activity and both Li P and Mn P activities,respectively. 关 键 词:biodegration lignin PEROXIDE manganese PEROXIDASE PEROXIDASE persistent organic POLLUTANT polysaccharide white ROT FUNGUS
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