NACC-1 regulates hepatocellular carcinoma cell malignancy and is targeted by miR-760

查看全文 作　　者：Linan [1]Yin;Tingting [2]Sun;Ruibao [1]Liu

机构地区：[1]Department of Interventional,Harbin Medical University Cancer Hospital,Harbin 150040,China;[2]Department of Gerontology,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China

出　　处：《Acta Biochimica et Biophysica Sinica》索引2020年第52卷第3期,共8页

基　　金：supported by the grants from the Haiyan Grant(No.JJMS 2016-6);the Scientific Research Project of Health Commission of Heilongjiang Province(No.2017-122). 摘　　要：Hepatocellular carcinoma(HCC)is the most prominent form of presentation in liver cancer.It is also the fourth most common cause of cancer-associated deaths globally.The role of nucleus accumbens associated protein-1(NACC-1)has been evaluated in several cancers.This protein is a transcriptional regulator that regulates a number of significant cellular processes.In the current study,we aimed to understand the role of NACC-1 in HCC.Primarily,we measured the expression of NACC-1 using quantitative real time polymerase chain reaction and western blot analysis.We knocked down the expression of NACC-1 in HCC cell lines Huh7 and HepG2 by transferring a commercially synthesized small interfering RNA and explored the impact of NACC-1 knockdown on cellular growth,migration,invasion,and chemoresistance to doxorubicin.Through bioinformatic analysis,we identified NACC-1 as a potential target of miR-760.Using a dual reporter luciferase assay,we confirmed the predicted target and assessed miR-760-mediated regulation of NACC-1 and rescue of tumorigenic phenotypes.We observed increased expression of NACC-1 in HCC.Furthermore,knockdown of NACC-1 resulted in reduced cell proliferation and invasion and increased susceptibility to doxorubicin-mediated chemosensitivity.Overexpression of miR-760 in HCC cell lines rescued NACC-1-mediated migration and invasion.We revealed that miR-760 regulated NACC-1 expression in HCC.Our data indicated that both miR-760 and NACC-1 could be used as prognostic markers,and miR-760 may have therapeutic benefits for HCC and other cancers. 关键词：NACC-1 HEPATOCELLULAR carcinoma miR-760 CHEMORESISTANCE

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NACC-1 regulates hepatocellular carcinoma cell malignancy and is targeted by miR-760

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