Direct cytoplasm delivery of gold nanoparticles for real-time apoptosis detection

查看全文 作　　者：Qiang [1]Li;Fengchao [1]Wang;Jie [1]Yang;Dingbin [1]Liu 机构地区：[1]Research Center for Analytical Sciences,Institute of Polymer Chemistry,State Key Laboratory of Medicinal Chemical Biology,and Tianjin Key Laboratory of Molecular Recognition and Biosensing,College of Chemistry,Nankai University,Tianjin,300071,China 出　　处：《Nano Research》索引2020年第13卷第3期,共8页 基　　金：the National Natural Science Foundation of China(No.21775075),the Fundamental Research Funds for Central Universities(China);the Thousand Youth Talents Plan of China. 摘　　要：Gold nanoparticles(AuNPs)assembled with fluorescent peptides through Au-S bonds(pep-AuNPs)have been widely used in biomolecular detection.However,due to the endo/lysosomal trapping after the nanoprobes enter cells,the direct delivery of AuNP probes into the cytoplasm for real-time imaging remains a difficult barrier for many cytoplasm-targeting agents.Here,we prepare AuNP@gel by wrapping a multi-functional nanogel structure on the surface of a single AuNP probe by in-situ polymerization in order to directly deliver AuNP probes into the cell cytoplasm.Compared with the pep-AuNP probes,which are trapped inside lysosomes for long periods,the AuNP@gel probes use the proton-sponge effect to effectively disrupt endo/lysosomal membranes and remain in the cytoplasm.In addition,the AuNP@gel probes rapidly escape from endo/lysosomes to avoid the complex environment that interferes with the stability of the AuNP probes and the lysosomal-storage trigger the upregulation of oxidative stress into the cells.The nanogel structure enables the AuNP probes to avoid some detrimental effects and to achieve high-fidelity fluorescence signals in the cells.Compared to traditional strategies for lysosomal escape,this one-step in-situ polymerization procedure avoids the complicated modification of additional ligands and is generally applicable to peptide-,DNA-,and polymerlinked AuNP probes. 关 键 词：gold nanoprobes NANOGEL endo/lysosomal escape real-time imaging apoptosis detection