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Exosomal piRNA profiling revealed unique circulating piRNA signatures of cholangiocarcinoma and gallbladder carcinoma

查看全文 作　　者：Xinjin [1]Gu;Chen [2]Wang;Hui [3]Deng;Chong [3]Qing;Rong [1]Liu;Sanhong [2]Liu;Xinying [3]Xue 高影响力作者 机构地区：[1]Departm ent of Hepatobiliary and Pancreatic Surgical Oncology,Chinese People's Liberation Army General Hospital,Medical School of Chinese People’s Liberation Army,Beijing 100853,China;[2]Shanghai Institute of Advanced Immunochem ical Studies,Shanghai Tech University,Shanghai 201210,China;[3]Departm ent of Respiratory and Critical Care,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China高影响力机构 出　　处：《Acta Biochimica et Biophysica Sinica》索引2020年第52卷第5期,共10页高影响力期刊 基　　金：This work was supported by the grants from the Clinical Research Fund of WU JIEPING Medical Foundation(No.320.6750.17293)and the Sanya Medical and Scientific Innovation Project(No.2018YW09). 摘　　要：Cholangiocarcinoma(CCA)and gallbladder carcinoma(GBC)are biliary tract cancers with poor five-year survival and high recurrence rates.Both CCA and GBC patients suffer from lack of circulating diagnostic biomarkers at the early stage.Extracellular vesicles,especially exosomes,have been emerged as promising diagnostic sources for cancers due to easy and quick accessibility.Hence,identification of exosomal biomarkers provides a novel strategy for CCA and GBC diagnosis.Here,five CCA patients and four GBC patients were enrolled for exosomal small RNA sequencing.Our data showed that exosomal piwi-interacting RNA(piRNA)populations were altered in the plasma of CCA and GBC patients.In comparison to healthy individuals,694 and 323 piRNAs were upregulated in CCA and GBC,respectively,while 36 and 191 piRNAs were down regulated.Interestingly,sequencing results predicted that piR-2660989,piR-10506469,piR-20548188,piR-10822895,piR-hsa-23209,and piR-18044111 were upregulated in both CCA and GBC plasma.Importantly,we further included blood samples from 50 health individuals,40 CCA patients,and 25 GBC patients and found that piR-10506469 were significantly increased in the exosomes of plasma from both CCA and GBC patients.Moreover,we analyzed the expression levels of differentially expressed exosomal piRNAs in the plasma of CCA and GBC patient before and after surgeries and found that piR-10506469 and piR-20548188 were significantly decreased in patients underwent surgeries.Taken together,our data revealed that exosomal piRNAs those are differentially expressed in CCA and GBC plasma may serve as potential biomarkers for the diagnosis of CCA and GBC. 关 键 词：EXOSOME PIRNA CCA GBC circulating diagnostic marker
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