Single-cell RNA sequencing identifies novel cell types in Drosophila blood

查看全文 作　　者：Yulong [1]Fu;Xiaohu [1,3]Huang;Peng [2]Zhang;Joyce van de [1,3]Leemput;Zhe [1,3]Han

机构地区：[1]Center for Precision Disease Modeling,Department of Medicine,University of Maryland School of Medicine,Baltimore,MD,21201,USA;[2]Divisions of Immunotherapy,University of Maryland School of Medicine,Baltimore,MD,21201,USA;[3]Division of Endocrinology,Diabetes and Nutrition,Department of Medicine,University of Maryland School of Medicine,Baltimore,MD,21201,USA

出　　处：《Journal of Genetics and Genomics》索引2020年第47卷第4期,共12页

基　　金：supported by grants from NIH to Z.H.(R01-HL134940,R01-DK098410)。 摘　　要：Drosophila has been extensively used to model the human blood-immune system,as both systems share many developmental and immune response mechanisms.However,while many human blood cell types have been identified,only three were found in flies:plasmatocytes,crystal cells and lamellocytes.To better understand the complexity of fly blood system,we used single-cell RNA sequencing technology to generate co mprehensive gene expression profiles for Drosophila circulating blood cells.In addition to the known cell types,we identified two new Drosophila blood cell types:thanacytes and primocytes.Thanacytes,which express many stimulus response genes,are involved in distinct responses to different types of bacteria.Primocytes,which express cell fate commitment and signaling genes,appear to be involved in keeping stem cells in the circulating blood.Furthermore,our data revealed four novel plasmatocyte subtypes(Ppn+,CAH7^+,Lsp^+and reservoir plasmatocytes),each with unique molecular identities and distinct predicted functions.We also identified cross-species markers from Drosophila hemocytes to human blood cells.Our analysis unveiled a more complex Drosophila blood system and broadened the scope of using Drosophila to model human blood system in development and disease. 关键词：DROSOPHILA BLOOD Single-cell RNA-seq Thanacyte Primocyte PLASMATOCYTE

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Single-cell RNA sequencing identifies novel cell types in Drosophila blood

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