Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences

查看全文 作　　者：Zhen [1]He;Tingting [1]Dong;Tielin [2]Wang;Wen [1]Chen;Xian [1]Liu;Liangjun [1]Li

机构地区：[1]School of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009,China;[2]State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Horticultural Plant Journal》索引2020年第6卷第5期,共8页

基　　金：Key project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302);the National Natural Science Foundation of China(Grant No.31601604);China Agriculture Research System(Grant No.CARS-24). 摘　　要：Lotus(Nelumbo nucifera Gaertn.)is an economically important perennial aquatic herb plant in China.Presently,viral diseases caused by Cucumber mosaic virus(CMV)and Dasheen mosaic virus(DsMV)are detected in lotus.In this study,the putatively novel badnaviruses from lotus plants(LBVs)were identified using next-generation sequencing of siRNAs and conventional Sanger sequencing based on the RT/RNase H coding region sequences.Lotus plants infected by LBVs harbored virus quasispecies.A unique recombination event of LBVs was found in a single lotus plant.Sap inoculation showed that LBVs could actively replicate in the lotus plants,but without a wide host range.The field survey of 43 lotus plants in Jiangsu Province showed a prevalence of 62.8%for LBVs,confirming that it is widely distributed in Jiangsu Province of China. 关键词：Nelumbo nucifera Badnaviruses mixed infection genetic variation

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Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences

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