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Detrimental effects of excessive fatty acid secretion on female sperm storage in chickens

查看全文 作　　者：Chaoliang [1,2]Wen;Chunning [1,2]Mai;Bin [1,2]Wang;Junying [1,2]Li;Congjiao [1,2]Sun;Ning [1,2]Yang 高影响力作者 机构地区：[1]Department of Animal Genetics,Breeding and Reproduction,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;[2]National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics,Breeding and Reproduction,Ministry of Agriculture and Rural Affairs,China Agricultural University,Beijing 100193,China高影响力机构 出　　处：《Journal of Animal Science and Biotechnology》索引2020年第11卷第3期,共13页高影响力期刊 基　　金：This work was supported by Programs for Changjiang Scholars and Innovative Research in Universities(IRT_15R62);China Agriculture Research Systems(No.CARS_40). 摘　　要：Background:Female sperm storage(FSS),the maintenance of sperm inside the female reproductive tract for an extended period of time,is pervasive among organisms with internal fertilization.Because FSS enables asynchronous mating and fertilization,it could be extremely important to reproduction.However,the physiological mechanisms underlying prolonged preservation and maintenance are poorly understood.Here,we used chicken,a typical oviparous animal,to determine the mechanisms ensuring sperm functionality in sperm storage tubules(SSTs).Results:We performed an insemination experiment on over two thousand hens at two periods,and found that the FSS capabilities varied widely among individuals.Except for the differences in the SST density between the two groups with distinct FSS abilities,we quantitatively profiled small-molecule metabolites derived from SST cells,and identified 28 metabolites with differential expression.In particular,high levels of lipids,fatty acids and lipid peroxidation product were observed in hens with low FSS capability.Pathway analysis showed that these differential metabolites were significantly enriched in the biosynthesis of unsaturated fatty acids.Moreover,we detected the total antioxidant capacity and lipid peroxidation level of SSTs,and found that chickens with a lower FSS ability had a significantly higher content of lipid peroxidation end-product,which was 2.4-fold greater than chickens with a higher FSS capability,and no significant difference was found in the total antioxidant capacity between these two groups.Conclusions:Our findings reveal that the long-term storage of sperm and the maintenance of their function in the female reproductive tract require an adequate microenvironment.The superabundance of fatty acids secreted by SST cells had detrimental effects on sperm storage in the female reproductive tract.Lipid peroxidation produces toxic biological substances that may cause irreversible damage to resident spermatozoa,resulting in short-term sperm retention and decreased fertility.Our findings provide new avenues for studying sperm storage and sustaining fertility. 关 键 词：CHICKENS Female sperm storage Lipid peroxidation Metabolomics Sperm storage tubules
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