维普中文期刊产品整合服务

Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant?

查看全文 作　　者：DOU Hai [1,2]Wei;TIAN Xiu [1,2]Jun;XIN De [1,2]Li;WEI [3]Ran;ZHOU [4]Wei;WANG [5]Hong;QIN Xuan [6]Guang;SHAO Jun [7]Yan;XU Bao [8]Ping;GE Li [7]Xia;SHI Da [9]Wei 高影响力作者 机构地区：[1]Beijing Tropical Medicine Research Institute,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;[2]Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Disease,Capital Medical University,Beijing 100050,China;[3]Qingdao Women and Children`s Hospital,Qingdao 266000,Shandong,China;[4]Department of Pediatrics,Peking University Third Hospital,Beijing 100191,China;[5]Department of Pediatrics,Civil Aviation General Hospital,Beijing 100123,China;[6]Department of Pediatrics,Beijing Chao-yang Hospital,Capital Medical University,Beijing 100043,China;[7]Department of Pediatrics,Emergency General Hospital(China Meitan General Hospital),Beijing 100028,China;[8]Department of Respiratory,Beijing Children’s Hospital,Capital Medical University,Beijing 100045,China;[9]Department of Pediatrics,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China高影响力机构 出　　处：《Biomedical and Environmental Sciences》索引2020年第33卷第12期,共9页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China[Grant No.81271890];Beijing Municipal Science&Technology Commission Grant[No.Z161100000116088 and Z1711000017081] 摘　　要：Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro. 关 键 词：CULTURE Macrolide resistance MLVA type Mycoplasma pneumoniae
相关文献

参考文献(50)

引证文献(12)

耦合文献(33)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费