Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells

查看全文 作　　者：Yu-Dong [1]Wei;Xiao-Min [1]Du;Dong-Hui [1]Yang;Fang-Lin [1]Ma;Xiu-Wei [1]Yu;Meng-Fei [1]Zhang;Na [1]Li;Sha [1]Peng;Ming-Zhi [2]Liao;Guang-Peng [3]Li;Chun-Ling [3]Bai;Wei-Shuai [4]Liu;Jin-Lian [1]Hua

机构地区：[1]College of Veterinary Medicine,Northwest A&F University,Shaanxi Centre of Stem Cells Engineering&Technology,Yangling,Shaanxi 712100,China;[2]College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China;[3]State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock,School of Life Sciences,Inner Mongolia University,Hohhot,Inner Mongolia 010021,China;[4]Department of Pathology,Yangling Demonstration Zone Hospital,Yangling Shaanxi 712100,China

出　　处：《Zoological Research》索引2021年第42卷第1期,共14页

基　　金：This work was supported by the China National Basic Research Program(2016YFA0100203);National Natural Science Foundation of China(31572399Detail,32072806,32072815,32002246);State Key Lab of Reproductive Regulation&Breeding of Grassland Livestock(SKL-OT-201801);Science and Technology Major Project of Inner Mongolia Autonomous Region of China(ZDZX2018065);and Shaanxi Province Science and Technology Innovation Team(2019TD-036);The authors thank Dr.John Clotaire Daguia Zambe for helpful comments about this paper,Jia Fang for the PGL3-NF-κB luciferase reporter plasmid,and Dong-Xue Che for bioinformatics analysis. 摘　　要：Double sex and mab-3-related transcription factor 1(Dmrt1),which is expressed in goat male germline stem cells(mGSCs)and Sertoli cells,is one of the most conserved transcription factors involved in sex determination.In this study,we highlighted the role of Dmrt1 in balancing the innate immune response in goat mGSCs.Dmrt1 recruited promyelocytic leukemia zinc finger(Plzf),also known as zinc finger and BTB domain-containing protein 16(Zbtb16),to repress the Toll-like receptor 4(TLR4)-dependent inflammatory signaling pathway and nuclear factor(NF)-κB.Knockdown of Dmrt1 in seminiferous tubules resulted in widespread degeneration of germ and somatic cells,while the expression of proinflammatory factors were significantly enhanced.We also demonstrated that Dmrt1 stimulated proliferation of mGSCs,but repressed apoptosis caused by the immune response.Thus,Dmrt1 is sufficient to reduce inflammation in the testes,thereby establishing the stability of spermatogenesis and the testicular microenvironment. 关键词：Male germline stem cells(mGSCs) GOAT DMRT1 PLZF Immune response

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Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells

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