Methodologies for preparation of prokaryotic extracts for cell-free expression systems

查看全文 作　　者：Stephanie [1]D.Cole;Aleksandr [1]E.Miklos;Abel [2,3]C.Chiao;Zachary [2,3]Z.Sun;Matthew [1,4]W.Lux

机构地区：[1]US Army Combat Capabilities Development Command Chemical Biological Center,8567 Ricketts Point Road,Aberdeen Proving Ground,MD,21010,USA;[2]Division of Biology and Biological Engineering,California Institute of Technology,Pasadena,CA,USA;[3]Synvitrobio Inc.,San Francisco,CA,USA;[4]8567 Ricketts Point Road,Bldg E3549 Room B140,APG,MD,21010,USA

出　　处：《Synthetic and Systems Biotechnology》索引2020年第5卷第4期,共16页

摘　　要：Cell-free systems that mimic essential cell functions,such as gene expression,have dramatically expanded in recent years,both in terms of applications and widespread adoption.Here we provide a review of cell-extract methods,with a specific focus on prokaryotic systems.Firstly,we describe the diversity of Escherichia coli genetic strains available and their corresponding utility.We then trace the history of cell-extract methodology over the past 20 years,showing key improvements that lower the entry level for new researchers.Next,we survey the rise of new prokaryotic cell-free systems,with associated methods,and the opportunities provided.Finally,we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible. 关键词：Cell-free systems Cell-free expression Cell-free extract Synthetic biology Methods

相关文献

参考文献（155）
引证文献（1）
耦合文献（0）

参考文献(155)

引证文献(1)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

参考文献(155)

引证文献(1)

用户提示