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In vitro antioxidant,anti-diabetic,cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C.maxima fruits

查看全文 作　　者：Arumugam [1]Abirami;Gunasekaran [1]Nagarani;Perumal [1]Siddhuraju 高影响力作者 机构地区：[1]Bioresource Technology Lab,School of Life Sciences,Department of Environmental Sciences,Bharathiar University,Coimbatore 641046,Tamil Nadu,India高影响力机构 出　　处：《Food Science and Human Wellness》索引2014年第3卷第1期,共10页高影响力期刊 基　　金：The authors wish to thank University Grants Committee(UGC), New Delhi, India (Grant number 34-259\2008) forfinancial assistance. 摘　　要：In the present study,antioxidant potential,α-amylase,α-glucosidase,cholinesterase and tyrosinase inhibitory activity of fresh juice from indigenous fruits of Citrus hystrix and C.maxima(Red&White var.)were investigated using an in vitro model.The contents of total phenolics,tannins,and total flavonoids ranged between 836.90 and 909.52 mg gallic acid equivalent(GAE)/L,333.33 and 523.21 mg gallic acid equivalent(GAE)/L and 224.88 and 262.22 mg rutin equivalent/L,respectively.The antioxidant activity of fresh juice was evaluated by employing different in vitro assays such as reducing power assay,DPPH•,ABTS•+and•OH radical scavenging capacities,peroxidation inhibition activity,antihemolytic assay.In addition,75.55%–79.75%ofα-amylase and 70.68%–72.83%ofα-glucosidase enzyme inhibition characteristics were found under in vitro starch digestion bioassay.Also,all the juice samples exhibited excellent tyrosinase(76.95%–80.79%),acetylcholinesterase(75.71%–79.74%)andβ-glucuronidase inhibitory activity(68.13%–69.38%).These results indicated that fresh juice of C.hystrix and C.maxima(Red&White var.)could be used as a source of antioxidant agents,functional food and nutraceuticals.©2014 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved. 关 键 词：Citrus fruits Fresh juice Antioxidant potential Enzymatic inhibitory activity NUTRACEUTICALS
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