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Screening the Best Material for Ploidy Identification of Sugarcane-related Genera

查看全文 作　　者：Lianying [1]MAO;Yiyun [1]GUI;Jinju [1]WEI;Haibi [1]LI;Ronghua [1]ZHANG;Hui [1]ZHOU;Xihui [1]LIU 高影响力作者 机构地区：[1]Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences/Sugarcane Research Center,Chinese Academy of Agricultural Sciences/Key Laboratory of Sugarcane Genetic Improvement in Guangxi,Nanning 530007,China高影响力机构 出　　处：《Agricultural Biotechnology》索引2021年第10卷第1期,共4页高影响力期刊 基　　金：Supported by National Natural Science Foundation of China(31860350);Special Fund for Basal Scientific Research of Guangxi Academy of Agricultural Sciences(GNK 2020YM24);Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(2021YT006)。 摘　　要：[Objectives] Flow cytometry is widely used to identify plant chromosome ploidy because of its simplicity, rapidity and accuracy. Chromosome ploidy identification is an important part of sugarcane ploidy breeding and application research. It is particularly important to find out the best detection part for ploidy identification in sugarcane. [Methods] The cell suspensions of sugarcane stem tips and leaves were prepared by blade chopping method. The cell suspensions were detected by flow cytometry. The best position for ploidy identification was determined by comparing the cell suspension prepared from stem tips and cell suspension prepared from leaves. [Results] The results showed that the cell suspension dissociated from stem tips was more clear than that from leaf cell suspension;the proportion of non-adherent cells in the suspension prepared from stem tips was larger than that from the leaf cell suspension;the main peak of the stem tip cell suspension was single and the number of cells was more than that of the stem tip cell suspension by flow cytometry. Using the known ploidy ‘Badilar’ as the internal reference, the ploidy of cyathomi 87-16 was detected to be 8.37. [Conclusions] Sugarcane shoot tips are an ideal material for ploidy identification. This study provides a theoretical basis for selecting the best detection site for ploidy identification of sugarcane. 关 键 词：Flow cytometry Chromosome ploidy SUGARCANE Cell suspension
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