In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

查看全文 作　　者：Muhammad [1,2,3,4]Shafiq;Yue [5]Zhang;Dashuai [2]Zhu;Zongxian [5]Zhao;Dong-Hwee [6]Kim;Soo Hyun [1,3,6]Kim;Deling [2]Kong

机构地区：[1]Division of Bio-Medical Science&Technology,KIST School,Korea University of Science and Technology,Seoul 02792,Republic of Korea;[2]State Key Laboratory of Medicinal Chemical Biology,Key Laboratory of Bioactive Materials of Ministry of Education,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),College of Life Science,Nankai University,Tianjin 300071,China;[3]Center for Biomaterials,Biomedical Research Institute,Korea Institute of Science and Technology,Cheongryang,Seoul 130-650,Republic of Korea;[4]Department of Chemistry,Center for Tissue Engineering&Regenerative Medicine,Pakistan Institute of Engineering&Applied Sciences(PIEAS),Nilore,Islamabad 45650,Pakistan;[5]Department of Physiology&Pathophysiology,Tianjin Medical University,Tianjin 300070,China;[6]KU-KIST Graduate School of Converging Science and Technology,Korea University,Seoul 02841,Republic of Korea

出　　处：《Regenerative Biomaterials》索引2018年第5卷第5期,共14页

基　　金：supported by the KIST Institutional Program and by the KUKIST Graduate School of Converging Science and Technology Program.Project supported by the National Science Foundation for Young Scientists of China(Grant No.81701839);The Youth Foundation of Tianjin Medical University(Grant No.2015KYZQ14). 摘　　要：Cardiovascular diseases cause huge socio-economic burden worldwide.Although a mammalian myocardium has its own limited healing capability,scaffold materials capable of releasing stem cell recruiting/engrafting factors may facilitate the regeneration of the infarcted myocardium.The aim of this research was to develop cardiac patches capable of simultaneously eluting substance P(SP)and insulin-like growth factor-1C(IGF-1C)peptide.Polycaprolactone/collagen type 1-based patches with or without SP and IGF-1C peptide were fabricated by co-electrospinning,which exhibited nanofibrous morphology.SP and IGF-1C/SP patches recruited significantly higher numbers of bone marrow-mesenchymal stem cells than that of the negative control and patch-only groups in vitro.The developed patches were transplanted in an infarcted myocardium for up to 14 days.Mice underwent left anterior descending artery ligation and received one of the following treatments:(i)sham,(ii)saline,(iii)patch-only,(iv)IGF-1C patch,(v)SP patch and(vi)IGF-1C/SP patch.SP and IGF-1C/SP patch-treated groups exhibited better heart function and attenuated adverse cardiac remodeling than that of the saline,patch-only and individual peptide containing cardiac patches.SP patch and IGF-1C/SP patch-treated groups also showed higher numbers of CD31-positive vessels and isolectin B4-positive capillaries than that of other groups.IGF-1C/SP-treated group also showed thicker left ventricular wall in comparison to the saline and patch-only groups.Moreover,IGF-1C/SP patches recruited significantly higher numbers of CD29-positive cells and showed less numbers of Tunel-positive cells compared with the other groups.These data suggest that SP and IGF-1C peptides may act synergistically for in situ tissue repair. 关键词：cardiac patch stem cell recruitment myocardial infarction ELECTROSPINNING

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In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

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