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Wet-spinning fabrication of shear-patterned alginate hydrogel microfibers and the guidance of cell alignment

查看全文 作　　者：You [1]Yang;Jing [1]Sun;Xiaolu [1]Liu;Zhenzhen [2]Guo;Yunhu [1]He;Dan [1]Wei;Meiling [1]Zhong;Likun [1]Guo;Hongsong [1]Fan;Xingdong [1]Zhang 高影响力作者 机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,Sichuan,Chengdu 610064,P.R.China;[2]Department of Gastroenterology,Hospital of the University of Electronic Science and Technology of China and Sichuan Provincial People’s Hospital,Sichuan,Chengdu 610072,P.R.China高影响力机构 出　　处：《Regenerative Biomaterials》索引2017年第4卷第5期,共9页高影响力期刊 基　　金：This work is supported by the National Natural Science Foundation of China(Contract Grant No.51473098,and 51673128). 摘　　要：Native tissue is naturally comprised of highly-ordered cell-matrix assemblies in a multi-hierarchical way,and the nano/submicron alignment of fibrous matrix is found to be significant in supporting cellular functionalization.In this study,a self-designed wet-spinning device appended with a rotary receiving pool was used to continuously produce shear-patterned hydrogel microfibers with aligned submicron topography.The process that the flow-induced shear force reshapes the surface of hydrogel fiber into aligned submicron topography was systematically analysed.Afterwards,the effect of fiber topography on cellular longitudinal spread and elongation was investigated by culturing rat neuron-like PC12 cells and human osteosarcoma MG63 cells with the spun hydrogel microfibers,respectively.The results suggested that the stronger shear flow force would lead to more distinct aligned submicron topography on fiber surface,which could induce cell orientation along with fiber axis and therefore form the cell-matrix dual-alignment.Finally,a multi-hierarchical tissue-like structure constructed by dual-oriented cell-matrix assemblies was fabricated based on this wet-spinning method.This work is believed to be a potentially novel biofabrication scheme for bottom-up constructing of engineered linear tissue,such as nerve bundle,cortical bone,muscle and hepatic cord. 关 键 词：tissue engineering WET-SPINNING cell-matrix alignment multi-hierarchical scaffold
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