Plasmid DNA-loaded asymmetrically porous membrane for guided bone regeneration

查看全文 作　　者：Se Heang [1]Oh;June-Ho [2]Byun;So Young [3]Chun;Young-Joo [1]Jang;Jin Ho [4]Lee

机构地区：[1]Department of Nanobiomedical Science,Dankook University,Cheonan 31116,Republic of Korea;[2]Department of Oral and Maxillofacial Surgery,Gyeongsang National University School of Medicine,Gyeongsang National University Hospital,Institute of Health Sciences,Gyeongsang National University,Jinju 52727,Republic of Korea;[3]BioMedical Research Institute,Kyungpook National University Hospital,Daegu 41404,Republic of Korea;[4]Department of Advanced Materials,Hannam University,Daejeon 34054,Republic of Korea

出　　处：《Journal of Materials Science & Technology》索引2021年第4期,共11页

基　　金：supported by grants from the Basic Science Research Program through the National Research Foundation(NRF)of Korea funded by the Ministry of Science&ICT(NRF-2017R1A2B2003848);the Bio&Medical Technology Development Program of the National Research Foundation(NRF)funded by the Ministry of Science&ICT(2019M3A9E2066347)。 摘　　要：Although bone defects can be restored spontaneously,bone reconstruction with sufficient strength and volume continues to be a challenge in clinical practices.In recent years,the use of a variety of biomaterials with bioactivity has been attempted to compensate for this limitation.Herein,we fabricated a pDNA(encoding for BMP-2)-loaded asymmetrically porous polycaprolactone(PCL)/Pluronic F127 membrane as a bioactive guided bone regeneration(GBR)membrane,using a modified immersion-precipitation method.It was observed that the GBR membrane allows continuous release of pDNA for more than20 weeks.The pDNA was sufficiently transfected into human bone marrow stem cells(h BMSCs)without significant cytotoxicity and the gene-transfected cells showed prolonged synthesis of BMP-2.From in vitro osteogenic differentiation and in vivo animal studies,the effective induction of osteogenic differentiation of h BMSCs and enhanced bone regeneration by the pDNA-loaded asymmetrically porous PCL/Pluronic F127 membrane was observed,suggesting that the pDNA-loaded membrane as a bioactive GBR membrane can be an alternative therapeutic technique for effective bone regeneration. 关键词：loaded POROUS ATTEMPTED

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Plasmid DNA-loaded asymmetrically porous membrane for guided bone regeneration

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